The Effect of Ischemic Preconditioning and Physical Exercise on the Endocrine Function of Skeletal Muscle - the Effect of CoQ10 Supplementation. In Vivo and in Vitro Studies.
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- The effects of IPC on physical performance
研究概览
简要总结
Recent studies have also shown that repeated episodes of ischemia, followed by reperfusion (IPC), can contribute to the development of adaptive changes not only in the area of the heart muscle, but also in the structure of the skeletal muscles.
In the project, several research questions will be evaluated e.g. what is the relationship between oxidative stress parameters, uric acid concentration and nitric oxide degradation products in groups of people undergoing two-week training in ischemic training, or what is the relationship between the expression of genes associated with muscle cell growth (e.g. myostatin gene) and the effect of ischemia preconditioning training etc.
详细描述
Both in sport and medicine, methods are constantly sought that can significantly contribute to improving the ability of tissues to perform their functions and protecting them from effort-induced damage.
In a wide range of interests, especially in people treated for ischemic changes of cardiovascular diseases, are protocols of tissue preconditioning by transient ischemia which significantly contribute to the increase in blood flow in tissue structure.
Recent studies have also shown that repeated episodes of ischemia, followed by reperfusion (IPC), can contribute to the development of adaptive changes not only in the area of the heart muscle, but also in the structure of the skeletal muscles. There is still a lack of detailed research on genetic conditions related to adaptive changes in this area of interest.
In the project, the following research questions were posed:
- What is the relationship between oxidative stress parameters, uric acid concentration and nitric oxide degradation products in groups of people undergoing two-week training in IPC training, or 2 weeks of CoQ10 supplementation in the light of non-training and non-supplementation?
- What is the relationship between two weeks of lower limb ischemia preconditioning training, or CoQ10 supplementation, and the expression of genes associated with the stress response (HSF-1, NF-kB, TNF dependent glass)?
- What is the relationship between the expression of genes associated with muscle cell growth (e.g. myostatin gene) and the effect of preconditioning training by ischemia?
- What is the relationship between the expression of genes associated with intracellular metabolism and the effect of CoQ10 supplementation?
- What is the relationship between the expression of genes encoding enzymes of energy pathways (e.g. GAPDH, LDH) and IPC training or CoQ10 supplementation in the study group?
- How does IPC training or CoQ10 supplementation affect the expression of genes responsible for membrane transport, and the Hedgehog pathway in muscle cells (including Gli1)?
- How does IPC training affect the expression of transcription factors: Hif-1-alpha (hypoxia-induced factor) and NF-kB and selected genes dependent on their activity?
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Single (Investigator)
入排标准
- 年龄范围
- 21 Years 至 29 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •gender (males only),
- •not taking medication during the study,
- •good health (no concomitant injuries),
- •negative history of cardiovascular disorders, autonomic nervous system, mental disorders, cerebrospinal injuries and other diseases that may directly affect the results obtained.
排除标准
- •Lack of consent to participate in the research project or negative result of medical examinations.
研究组 & 干预措施
Supplemented population 300mg
Supplemented population 300mg/24h CoQ10 - 25 participants
干预措施: CoQ10 supplementation (Dietary Supplement)
Training and IPC preconditioning
Group subjected to training and preconditioning by ischemia (IPC training) - 25 participants,
干预措施: Ischemic preconditioning training (Procedure)
Control group (placebo)
Control group to CoQ10 supplementation (placebo)- 25 participants
干预措施: Sham IPC combined with placebo (Dietary Supplement)
IPC control group
IPC control group - 25 participants
干预措施: IPC training combined with CoQ10 supplementation (Dietary Supplement)
结局指标
主要结局
The effects of IPC on physical performance
时间窗: 8 weeks
IPC training will be combined with endurance exercise and VO2max and serum Lactate will be measured
The effects of CoQ10 supplementation on physical performance
时间窗: 8 weeks
CoQ10 supplementation combined with endurance training will be evaluated for endurance improvement in young men. VO2max, myokines, genes associated with the stress response will be measured
The effects of CoQ10 supplementation and IPC on physical performance
时间窗: 8 weeks
CoQ10 supplementation combined with IPC and endurance training will be evaluated for endurance improvement in young men. VO2max, myokines, genes associated with the stress response, ulic acid will be measured
次要结局
未报告次要终点