mTOR and Adipose Tissue Inflammation in Humans
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 80
- 主要终点
- mTORC2 activity in adipose tissue measured by immunoblot
研究概览
简要总结
The target of rapamycin complex 2 (TORC2) is an evolutionarily conserved serine/threonine protein kinase that controls growth and metabolism. In mammals (including humans), mammalian TOR complex 2(mTORC2) contains mammalian TOR (mTOR), RICTOR, mSIN1 protein, and mLST8 gene. In an animal model, the adipose-specific rictor knockout (AdRiKO) mouse, systemic insulin resistance, hepatic steatosis, and cardiovascular dysfunction develop upon high fat diet (HFD)-induced obesity or aging. To find a molecular link between adipose mTORC2 and systemic insulin resistance, investigators have already performed transcriptomics and proteomics analysis on visceral white adipose tissue in a mouse model. The aim of the study is to confirm a molecular link between adipose mTORC2 and systemic insulin resistance in humans.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Obese volunteers (BMI > 35kg/m2) aged 18 to a maximum of 60 years, males and females; insulin-resistant, scheduled for elective bariatric surgery.
- •Normal weight patients (BMI < 27kg/m2) aged 18- max. 60 years, males and females; insulin-sensitive, scheduled for elective surgery.
排除标准
- •Known diabetes mellitus
- •Chronic inflammatory disease (inflammatory bowel disease, rheumatoid disease, cancer)
- •Acute inflammatory disease
- •Known renal disease: kidney failure
- •Pregnancy:
- •history of gastrointestinal surgery with major changes to the gastrointestinal tract (removal of stomach, any GI-bypass)
- •Substance abuse, alcohol abuse
- •Inability to follow procedures due to psychological disorders, dementia
- •insufficient knowledge of project language (German)
结局指标
主要结局
mTORC2 activity in adipose tissue measured by immunoblot
时间窗: single time point at surgery
measurement of mTORC2 activity in adipose tissue once at surgery
次要结局
- Mcp1 messenger ribonucleic acid (mRNA) level in adipose tissue measured by quantitative polymerase chain reaction (PCR)(single time point at surgery)