Aging Subcutaneous Adipose Tissue Response to Overfeeding
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 2
- 主要终点
- Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+)
研究概览
简要总结
The purpose of this pilot study is to determine whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. The investigators hypothesize that the adipose tissue response to overfeeding may be weakened in older when compared to younger men, thereby leading to worsened ectopic storage depots for older men.
详细描述
This pilot study will begin to address whether adipose tissue biology changes with advancing age. Specifically, we will test whether adipose tissue loses some of its ability to expand in response to overfeeding in older compared to younger men. To test this possibility the Investigators will recruit overweight and mildly obese older (age 55-75y) and younger (age 25-40y) men and collect adipose tissue biopsies before and after 7 days of overfeeding. The investigators will test how well the cells in adipose tissue expand in response to overfeeding and whether this differs between younger and older men.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 25 Years 至 75 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Aged 25-40y OR 55-75y
- •BMI 28-35 kg/m2
- •Weight stable (±2kg over past 3 mo)
- •Sedentary to moderately active (<3 d/wk of exercise lasting >20min)
- •Non-smokers (never used or quit >7yrs prior)
排除标准
- •Currently using hormones, glucose or lipid lowering medication
- •Engaged in exercise or diet program
- •Currently gaining or losing weight or using weight loss drugs
- •Diabetes mellitus (diagnosis, treatment, FBG ≥126 mg/dL)
- •Uncontrolled metabolic disorders (e.g., thyroid)
研究组 & 干预措施
MA
Middle-aged overweight and obese men (age 25-40yr)
干预措施: Overfeeding (Other)
OA
Older overweight and obese men (age 55-75yr)
干预措施: Overfeeding (Other)
结局指标
主要结局
Change in adipose-derived stem cell (ASC) differentiation (Delta%oil red O+)
时间窗: 1 wk of overfeeding
age-related difference in the ASC differentiation response to overfeeding
次要结局
- Change in ASC proliferation (Delta rate of cell growth)(1 wk of overfeeding)
- Change in expression of C/EBPalpha, C/EBPbeta, PPARgamma2, SREBP1c, SREBf1a, and Wnt10b (fold-changes in mRNA expression)(1 wk overfeeding)
- Change in intramyocellular (IMCL) di-saturated diacylglycerol (DAG) localization (Delta %membrane:cytosol)(1 week overfeeding)