Antimicrobial Peptides in Periodontitis : a Pilot Study
概览
- 阶段
- 不适用
- 干预措施
- 未指定
- 疾病 / 适应症
- Periodontal Disease : Chronic Periodontitis
- 发起方
- CHU de Reims
- 入组人数
- 29
- 试验地点
- 1
- 主要终点
- Antimicrobial peptides expression levels
- 状态
- 已完成
- 最后更新
- 3个月前
概览
简要总结
Periodontal diseases are complex chronic pathologies presenting a high prevalence worldwide. Etiopathological process involved in initiation and progression of these multifactorial diseases are not well understood, explaining the huge clinical forms variability and the complexity of the currently used classification of periodontal diseases. Recently, the implication of antimicrobial peptides (AMPs) have been suggested as potential track of explanation for periodontal diseases pathogeny. The here presented study explore the level of expression of genes coding those peptides by studying periodontal smears obtained from healthy and periodontitis patients.
详细描述
This study explore the level of expression of genes coding those peptides by studying Messenger RNA (mRNA) purified from periodontal smears obtained from healthy and periodontitis patients by "Masterpure©" protocol.After reverse transcription (RT-PCR), in-silico design of primers and specificity and efficiency calculation, Complementary DNA (cDNA) are quantified by a semi-quantitative method (delta-delta CT) vs a Housekeeping gene. Wilcoxon-Mann \& Whitney tests and Chi² tests are performed to check the significativity of the results between groups.
研究者
入排标准
入选标准
- •Patients suffering of moderate to severe chronic periodontitis as described by the classification Armitage-1999, aged 35-65yo
排除标准
- •Pregnant females, patients suffered from any other systemic diseases (cardiovascular, pulmonary, liver, cerebral, diseases or diabetes, cancer)
- •patients that had received antibiotic treatment in the previous 3 months or who were taking long-term anti-inflammatory drugs; who had received a course of periodontal treatment or orthodontics treatment within the last 6 months; who were heavy smokers (\>5 cig/day)
结局指标
主要结局
Antimicrobial peptides expression levels
时间窗: Day 0
Level of expression of antimicrobial peptides assessed by RT-PCR
次要结局
- mRNA quantity(Day 0)