Purine-Analog-Containing Non-Myeloablative Allogeneic Stem Cell Transplantation for Treatment of Hematologic Malignancies and Severe Aplastic Anemia
试验速览
- 阶段
- 2 期
- 状态
- 已完成
- 入组人数
- 58
- 试验地点
- 2
- 主要终点
- Evaluation of Donor Engraftment
研究概览
简要总结
RATIONALE: Drugs used in chemotherapy use different ways to stop cancer cells from dividing so they stop growing or die. Peripheral stem cell transplantation may be able to replace immune cells that were destroyed by chemotherapy used to kill cancer cells.
PURPOSE: Phase II trial to study the effectiveness of combination chemotherapy followed by peripheral stem cell transplantation in treating patients who have hematologic cancer or aplastic anemia.
详细描述
OBJECTIVES:
- Determine the rates of durable full donor hematologic engraftment in patients with high-risk hematologic malignancies or severe aplastic anemia treated with non-myeloablative conditioning using fludarabine, cyclophosphamide, and anti-thymocyte globulin followed by allogeneic peripheral blood stem cell transplantation.
- Determine the acute and delayed toxic effects of this non-myeloablative conditioning regimen in this patient population.
- Determine the event-free and overall survival of patients treated with this regimen.
- Determine the incidence and severity of acute and chronic graft-versus-host disease in patients treated with this regimen.
- Determine the rate and quality of immune reconstitution in patients treated with this regimen.
- Determine the rate of disease relapse and incidence of post-transplantation lymphoproliferative disease in these patients.
OUTLINE: Patients are stratified according to disease category (malignant vs non-malignant) and graft source (unrelated vs HLA-matched sibling).
Beginning at least 4 weeks after conventional-dose chemotherapy, patients receive non-myeloablative conditioning comprising fludarabine IV over 30 minutes on days -8 to -4, cyclophosphamide IV over 2 hours on days -3 to -2, and anti-thymocyte globulin IV over at least 4 hours on days -2 and -1. Patients undergo filgrastim (G-CSF)-mobilized allogeneic peripheral blood stem cell transplantation on day 0.
Patients are followed weekly for 3 months, every 2 weeks for 3 months, monthly for 6 months, and then every 2 months thereafter.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- — 至 70 Years（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •DISEASE CHARACTERISTICS:
- •Histologically proven high-risk hematologic malignancy
- •Acute non-lymphocytic leukemia (ANLL) after induction failure, or in first complete remission (CR) with high-risk features, including any of the following:
- •Stem cell or biphenotypic classification (AML-M0)
- •Erythroleukemia (AML-M6)
- •Acute megakaryocytic leukemia (AML-M7)
- •Cytogenetic markers indicative of poor prognosis
- •Failure to achieve CR after standard induction therapy
- •Acute lymphocytic leukemia (ALL) or ANLL in relapse or second or subsequent remission
- •Chronic myelogenous leukemia (CML) in chronic or accelerated phase
- •Patients with CML in blast crisis are eligible after reinduction chemotherapy places them in chronic phase
- •High-risk ALL in first CR with high risk defined by presence of t(4;11), t(9;22) translocation, hyperleukocytosis (initial WBC greater than 30,000/mm^3), or failure to achieve CR by day 28 after standard induction
- •No T-cell ALL or t(8;14) positive B-cell ALL in first remission with hyperleukocytosis
- •Myelodysplastic syndrome by peripheral blood smear and bone marrow examination
- •Refractory to medical management OR
- •Cytogenetic abnormalities predictive of transformation into acute leukemia including 5q-, 7q-, monosomy 7, and trisomy 8 OR
- •Evidence of evolution to AML (e.g., refractory anemia with excess blasts (RAEB) or RAEB in transformation)
- •Multiple myeloma, non-Hodgkin's lymphoma (NHL), ANLL, or ALL with recurrent disease after autologous stem cell transplantation (SCT)
- •At least 3 months since prior autologous SCT
- •Hodgkin's lymphoma, NHL, or multiple myeloma beyond first CR or primary induction failures whose disease has demonstrated sensitivity to pre-transplantation cytoreduction (defined as greater than 50% reduction in tumor burden)
- •Mantle zone NHL allowed after induction therapy
- •Myeloproliferative disorder that is non-responsive to medical management and requires allografting, unless evidence of grade 3 or worse myelofibrosis on marrow biopsy OR
- •Histologically proven acquired severe aplastic anemia (SAA) that is recurrent or unresponsive after anti-thymocyte globulin and/or cyclosporine
- •SAA defined by at least 2 of the following conditions:
- •Granulocyte count less than 500/mm^3
- •Platelet count less than 20,000/mm^3
- •Absolute reticulocyte count less than 20,000/mm^3 after correction for hematocrit
- •Ineligible for full ablative conditioning due to any of the following conditions:
- •Prior extensive therapy (more than 2 salvage chemotherapy regimens and/or autologous transplantation)
- •Over age 55 OR
- •Under age 55 with comorbid disease (e.g., suboptimal cardiac, pulmonary, or renal function and/or prior life-threatening infection)
- •HLA-A, B, and DR phenotypically identical sibling donor OR
- •HLA-A, B, and DR identical genetically matched unrelated donor
- •No ANLL in first CR (less than 5% blasts in marrow) with translocations t(8;21) and inv(16) unless failed first-line induction therapy OR
- •No ANLL in first CR (less than 5% blasts in marrow) with translocations t(15;17) abnormality unless failed first-line induction therapy OR molecular evidence of persistent disease
- •No active CNS disease
- •PATIENT CHARACTERISTICS:
- •Performance status:
- •Zubrod 0-1
- •Karnofsky 80-100%
- •Life expectancy:
- •At least 3 months
- •Hematopoietic:
- •See Disease Characteristics
- •ALT/AST no greater than 4 times normal
- •Bilirubin no greater than 2.0 mg/dL
- •See Disease Characteristics
- •Creatinine clearance at least 50 mL/min
- •Cardiovascular:
- •See Disease Characteristics
- 另有 22 项未显示
排除标准
- 未提供
结局指标
主要结局
Evaluation of Donor Engraftment
时间窗: at 28 days
Peripheral blood from the donor and patient is obtained for chimerism studies. The primary analysis will consist of estimating the graft failure proportions for each of the separate patient groups and calculating confidence intervals for these proportions. This analysis will be done conditional on patients surviving at least 28 days.
次要结局
- Stable donor hematopoietic chimerism(at day 100)
- Event free and overall survival(to progression/death)