Investigating the Bioavailability of Bioactive Compounds of Vegetable (carrot) in Human Bio-fluids After Consumption
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 8
- 试验地点
- 1
- 主要终点
- Absorption of PA and carotenoids
研究概览
简要总结
Carrot is a unique vegetable owing to the availability of bioactive compounds polyacetylenes (PA) as well as carotenoids present in it. These compounds have been shown to be associated with reduced cancer risk in epidemiological-based studies, and also showed anticancer properties in vitro studies and in vivo in mouse and rat models. To investigate whether the PA and carotenoids could have beneficial effects in humans, the present study is designed to determine whether they are detectible in human biofluids after the ingestion of a portion of carrots and whether there is a difference in the magnitude of detection after eating a large (300g) and normal (100g) portion.
详细描述
The occurrence of PA and carotenoids in carrot is not a guaranteed that they have potential health benefits and biological activity in humans, as there is still insufficient knowledge as to whether they can be absorbed in human bio-fluids (blood, urine and faeces) after the food (carrot) is consumed in the literature. The bioavailability of compounds depends upon both the chemical forms and physical structures which enable a certain proportion of such compounds into circulation when introduced into the body and so are able to have an active effect.
The main goal of this trial is to investigate determine the fate of (PAs and carotenoids) found in carrots human plasma, urine and faeces.
To achieve this goal, health adult volunteers will be instructed to restrict their diet (To avoid eating food that contain the compounds of interest) for 48hrs and complete 24hr food diary.
They will fast overnight and collect urine and faecal samples on the morning of the test session. Once they arrive in the research facility, they will have fasted blood taken before eating a portion of carrots (either 100 or 300g) with bread and butter. Further blood samples will be taken at 1, 2, 4, 6, 8 and 24 hours. Urine sample will be collected at 4, 8, 12 and 24hr and up to 48hr after the carrot consumption, and faecal samples will be collected at any time the participants feel bowel movement during the study duration up to 48hr, noting the time when each sample was generated.
The participants will attend two test sessions and will be randomised to receive high (300g) and normal (100g) doses of carrot. The washout period separating the two testing sessions is at least a week.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Screening
- 盲法
- Single (Investigator)
入排标准
- 年龄范围
- 20 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy adults 20+ years old.
- •Having body mass index (BMI) between ≥18.5 - ≤30 kg/m
- •Must be willing to eat a portion (100g) of carrot in the test session 1, alongside 2 slices of bread with 10g butter.
- •Must be willing to eat a portion (300g) of carrot in the test session 2, alongside 2 slices of bread with 10g butter.
排除标准
- •Having any metabolic or gastrointestinal conditions.
- •Having allergies to carrot or bread or butter.
- •Taking any medication that affects metabolism or digestion.
研究组 & 干预措施
High dose (300g) carrot with 2 slices of bread and 10g butter
Test Session 2:
The participants will consume the 300g carrots with bread and butter. This is considered a reasonable high enough dose that detection of the compounds must occur. This dose would be compared with the normal dose (100g) to see if there is more absorption of the compound when eaten a hiher dose.
干预措施: Carrots (Dietary Supplement)
Experimental: Normal dose (100g) carrot with 2 slices of bread and 10g butter
Test session 1: This is a normal dose (100g) which would be compared with the high dose to see if there dose-dependent effect in the magnitude of absorption.
Test Session 2: All the process as in the test session 1 will be repeated for the 300g dose carrot.
干预措施: Carrots (Dietary Supplement)
结局指标
主要结局
Absorption of PA and carotenoids
时间窗: 48hr
The primary outcome is testing whether a change in concentration of PA and carotenoids is seen between baseline and various time points in the biofluids after ingesting carrots.
次要结局
- Dose-effect between the absorption of PA and carotenoids from 100g and 300g carrots(48hr)