Long-term, Substantial Weight Loss and Insulin Regulation of Lipolysis
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- Mayo Clinic
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- insulin regulation of lipolysis (IC50)
研究概览
简要总结
It is not known how much improvement in insulin regulated lipolysis (the breakdown of triglycerides) occurs following substantial, sustained weight loss. Researchers will test the effects of inflammation and lipolysis regulation in people before and after bariatric surgery (sleeve gastrectomy) to answer these questions.
详细描述
The purpose of this study is to determine whether the adipose inflammatory cell and cytokine content in Class III obesity is related to lipolysis insulin resistance and, if so, whether sustained, substantial weight loss one year following bariatric surgery reduces inflammation in parallel with improved insulin regulation of lipolysis
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •BMI 40 - 50 kg/m
- •Mo active physical illness that would interfere with mobility or weight loss after bariatric surgery
排除标准
- •Type 1 or Type 2 Diabetes diagnosis or fasting plasma glucose ≥126 mg/dL
- •Active coronary artery disease
- •Participation in structured exercise (>2 times per week for 30 minutes or longer)
- •Medications known to affect adipose tissue metabolism (e.g., beta blockers, corticosteroids)
- •Renal insufficiency (serum creatinine > 1.5mg/dl)
- •Chronic active liver disease (Bilirubin > 17mmol/L, AST > 144 IU/L, or ALT>165IU/L)
- •Pregnancy or breastfeeding.
研究组 & 干预措施
Gastric sleeve surgery
before and after weight loss from bariatric surgery
干预措施: Gastric sleeve/bariatric surgery (Procedure)
结局指标
主要结局
insulin regulation of lipolysis (IC50)
时间窗: Before bariatric surgery and approximately 1 year after surgery
The ability of insulin to suppress FFA palmitate release will be assessed using a 2-step insulin clamp and isotope dilution techniques for palmitate kinetics
adipose macrophage content, adipose IL-6 and TNF gene expression
时间窗: Before bariatric surgery and approximately 1 year after surgery
Adipose tissue macrophage content as assessed by immunohistochemistry using stains for CD36, CD14 and CD206 will be done. mRNA expression for IL-6 and TNF will be measured
次要结局
- adipocyte perilipin 1 and HSL and ATGL(Before bariatric surgery and approximately 1 year after surgery)
研究者
Michael D. Jensen
Principal Investigator
Mayo Clinic