sTudy of the bioEnergetics and fAtigue Responses of a Basketball gaMe: The TEAM Trial
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Measurement of countermovement jump height
研究概览
简要总结
The aims of the study are:
- To examine skeletal muscle and blood metabolites during an official basketball game and to relate those to skeletal muscle performance during and after the game.
- To investigate whether skeletal muscle and blood metabolites are related to game-induced fatigue.
- To relate field activity during a basketball game with the responses of skeletal muscle and blood metabolites.
- To investigate how blood lactate reflects muscle lactate concentration during a basketball game.
- To determine whether temporary fatigue develops during a basketball game. game.
详细描述
The basketball players will participate in three experimental games (N = 10/game) on an indoors basketball stadium. During a 2-week familiarization period, volunteers will be familiarized with the experimental procedures and participate in very light training (at local basketball facilities) aimed at developing match tactics and team cohesion. At the end of the familiarization period and before the experimental game, participants will undergo a 3-day baseline performance testing (resting conditions) at University (Department of Physical Education and Sport Sciences, University of Thessaly) facilities. Prior to the games, the players will refrain from strenuous exercise and intake of alcohol for 48 hours and from tobacco and caffeine for 12 h. Dietary intake will be monitored with daily diet recalls for 7 days before the experimental period and will be standardized for all participants by a registered dietitian. The dietitian will also prescribe the meals and snacks on the game-day. On the day of the game, participants will arrive at the stadium 1.5 h before for resting skeletal muscle and blood sampling. For the first muscle biopsy and in preparation for later obtainment of biopsies from vastus lateralis muscle. An incision will be made under local anesthesia (20 mg/ml xylocaine without adrenalin) and covered by sterile band-aid strips and a thigh and shoulder bandages. In addition, an inertial measurement unit (IMU) and a heart rate monitor will be placed on each subject. During the match, a number of physiological measurements will be performed at fixed times (before the game, at half-time and post-match) and after intense exercise periods within the four 10-min periods of the game. Volunteers will be randomly assigned, using the sealed envelope procedure, in six teams representing all field positions and each team will play in one of three experimental games (random allocation using standard procedures) against each other in a full 40-minute game according to official regulations. A total of 10 additional players (from various playing positions) will serve as substitute players that could enter the pitch for a few minutes when a player will have to leave the court (either participate in measurements or will have completed it's pre-determined playing time or in case of an injury). Fifteen players (pre/post game measurement group) will have muscle and blood samples taken at rest, at the end of the 2nd period (half-time) and at the end of the 4th period (end of game). A second group of eight players (intense periods measurement group 1) will have muscle and blood samples collected at rest and during the first and third period of the game (immediately after an intense period of game-play). A third group of seven players (intense periods measurement group 1) will have muscle and blood samples collected at rest and during the second and fourth period of the game (immediately after an intense period of game-play). An intense exercise period of game-play is identified as a period with a heart rate of > 90% of maximal [as assessed by the maximal heart rate obtained during a graded exercise test to measure the maximal oxygen consumption (VO2max)], a high number of high-intensity runs (speed of > 18 km/h), sprints (speed of > 25 km/h) and/or jumps evaluated by a professional licensed coach. All muscle biopsies will be sampled from the vastus lateralis. All players will have their field activity and heart rate measured throughout the game using IMU units and heart rate monitors. Games will start at 17.00 - 18 p.m. to avoid diurnal variations and will be held under normal environmental conditions. A standardized 30-min warm-up period will be performed before each game. All games will be videotaped.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Health status: were free of recent musculoskeletal injuries and/or illnesses (≥ 8 months before the study).
- •Not consuming ergogenic supplements/medications (≥ 6 months before the study)
- •Not smokers
排除标准
- •Completion of the pre-determined participation time during the experimental game.
- •Completion of all field measurements.
- •Completion of all pre-determined sampling points for biological tissue.
研究组 & 干预措施
BASKETBALL GROUP
干预措施: BASKETBALL GAME (Other)
结局指标
主要结局
Measurement of countermovement jump height
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be measured using a force platform system
Bilateral strength peak force and rate of force development on mid-thigh pull
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be measured using a dynamometer.
Measurement of maximal sprinting speed over 5 meters
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
5-m sprinting speed will be assessed on the basketball court using infrared photocells
Measurement of maximal sprinting speed over 20 meters
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
20-m sprinting speed will be assessed on the basketball court using infrared photocells
Measurement of the capacity to perform repeated sprints
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
5 x 20m repeated maximal sprints will be performed with 25 sec rest in between. Speed will be recorded using infrared photocells
Measurement of field activity variables during the basketball game
时间窗: Continuous monitoring during each 40-minute game.
Field activity will be continuously recorded during the basketball game using a microsensor inertial measurement unit (Kinexon Perform IMU).
Measurement of heart rate during the basketball game ad recording the average and maximal heart rate of the game.
时间窗: Continuous monitoring during each 40-minute game.
Field activity will be continuously recorded during the basketball game using a Team Polar Pro heart rate monitor.
Measurement of skeletal muscle glycogen content
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be assessed on muscle biopsy samples. Muscle glycogen content will be determined spectrophotometrically.
Measurement of skeletal muscle fiber-type distribution and fiber-type- specific glycogen content.
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be assessed on muscle biopsy samples by histochemical analysis
Measurement of skeletal muscle phosphocreatine levels
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be assessed on muscle biopsy samples by a flurometric assay.
Measurement of skeletal muscle lactate
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be assessed on muscle biopsy samples by a flurometric assay.
Measurement of effort and fatigue
时间窗: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be measured by using the Borg Rating of Perceived Exertion (RPE) scale
次要结局
- Body mass(Change from baseline (pre-game) to 40 minutes of the game)
- Body height(At baseline)
- Body mass index (BMI)(At baseline)
- Body fat (BF)(At baseline)
- Fat mass (FM)(At baseline)
- Fat-free mass (FFM)(At baseline)
- Bone mass density(At baseline)
- Bone mass content(At baseline)
- Dietary intake(At baseline)
- Evaluation of physical activity (PA)(At baseline)
- Rest Heart Rate(At baseline)
- Peak Maximal oxygen consumption (Peak VO2)(At baseline)
- Basketball-specific conditioning (endurance)(At baseline)
- Basketball-specific conditioning (recovery)(At baseline)
- Concentric and eccentric isokinetic peak torque of knee extensors and flexors of both dominant and non-dominant limbs(At baseline)
- Maximal heart rate(Change from baseline (pre-game) to that recorded during the 40-minute game)
- Measurement of blood lactate(Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).)
- Measurement of plasma glucose concentration(Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).)
- Measurement of blood Glycerol concentration(Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).)
- Measurement of Plasma ammonia concentration(Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).)
- Measurement of Plasma potassium concentration(Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).)
- Measurement of Plasma- free fatty acid(Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).)
- Measurement of blood hemoglobin and hematocrit(Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).)
- Measurement of fluid loss and intake(Change from baseline (pre-game) to 40 minutes of the game)
研究者
Ioannis G. Fatouros
Professor
University of Thessaly