Regulatory Network and Diagnostic Value of Key Autophagy-related Genes in Sepsis
试验速览
- 阶段
- 不适用
- 入组人数
- 40
- 主要终点
- Diagnostic Value of Key Autophagy-related Genes in Sepsis
研究概览
简要总结
Autophagy plays an important role in the occurrence and development of sepsis. This study aims to explore and verify the key autophagy-related genes in sepsis, then construct their regulatory networks and evaluate their potential diagnostic value, so as to provide new ideas for the diagnosis and treatment of sepsis.
详细描述
(1) Sepsis-related datasets GSE65682,GSE134347 and GSE134358 were downloaded from GEO database, and DEmRNA,DElncRNA and DEmiRNA were obtained by differential analysis. (2) Autophagy related genes (ARGs) were obtained from Human Autophagy Database, and ARGs in GSE65682 and GSE134347 were extracted. DEARGs were obtained by differential analysis, and GO and KEGG enrichment analysis were performed. (3) Sepsis-related genes were obtained by WGCNA analysis, and key DEARGs in sepsis were obtained by intersection with DEARGs, and then transcription factors and ceRNA regulatory network were analyzed. (4) Immune infiltration analysis was used to evaluate the distribution of immune cells in the blood of patients with sepsis, and its correlation with key DEARGs was further analyzed. (5) The diagnostic functions of key DEARGs were analyzed. (6) Peripheral blood samples from sepsis patients and healthy controls were collected and verified by RT-qPCR.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •over 18 years old; 2) Meeting the diagnostic criteria of Sepsis 3.0; 3) Hospital stay longer than 24 hours.
排除标准
- •Malignant tumor; 2) Autoimmune diseases; 3) Use of immunosuppressants in the past two weeks; 4) Readmission or transfer from another ICU due to the same disease.
结局指标
主要结局
Diagnostic Value of Key Autophagy-related Genes in Sepsis
时间窗: October 2022
The diagnostic functions of key DEARGs (Differential Autophagy-related Genes) were analyzed
Regulatory Network of Key Autophagy-related Genes in Sepsis
时间窗: October 2022
Key DEARGs in sepsis were analyzed for transcription factors and ceRNA regulatory network
Verified Key Autophagy-related Genes in Sepsis by RT-qPCR.
时间窗: December 2022
Peripheral blood samples from sepsis patients and healthy controls were collected and verified by RT-qPCR.
次要结局
未报告次要终点