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临床试验/NCT02380846
NCT02380846已完成不适用

The Metabolic Effects of Consuming Carbohydrate With Different Protein Types

Clinical Nutrition Research Centre, Singapore0 个研究点目标入组 15 人开始时间: 2013年9月1日最近更新:
适应症
干预措施

试验速览

阶段
不适用
状态
已完成
发起方
入组人数
15
主要终点
Blood glucose response (incremental area under the curve) measured using the hemocue

研究概览

简要总结

This study aims to find out how consuming different proteins with rice affect metabolism. Most meals the investigators eat predominantly consist of a carbohydrate and protein (i.e. rice, noodles, bread etc with meats or seafood). However, it is still unknown how consuming different proteins with commonly eaten carbohydrates affect metabolism. Previous studies have shown that proteins stimulate hormones such as insulin, glucagon and gut hormones. However, the extent of the response depends on protein type. The metabolic responses to carbohydrates have also been shown to be greatly affected when they are eaten with proteins. However, most of the previous studies have used glucose as the carbohydrate and it is still unknown how eating proteins with carbohydrate foods such as rice affect metabolism. Therefore, this study has been initiated to determine the metabolic effects of eating different protein types with rice. Using the most common carbohydrate eaten in Asia (rice) and four commonly eaten protein foods (egg, chicken, fish and beancurd), this study aims to observe the metabolic effects of co-ingesting proteins and carbohydrate. The resulting data will provide valuable insights into the metabolic effects of protein-carbohydrate meals and will be useful in the development of practical advice and dietary guidelines for those with chronic diseases (such as diabetes and obesity).

详细描述

The study will recruit 20 healthy Chinese male subjects from the general public over a period of one year. The test foods will be rice, rice with egg, rice with fish, rice with chicken and rice with beancurd.Potential participants will have to come on one morning in an overnight fasted state for consenting and screening procedures. Only those fulfilling the inclusion and exclusion criteria will be admitted into the study. Each participant will be required to come on 5 non-consecutive days, with each session lasting approximately 4 hours. On each day, participants will arrive at the laboratory between 8-9 am following an overnight fast. An indwelling catheter will be inserted into a vein in their forearm or antecubital fossa and kept patent. Baseline blood samples will be obtained after which they will be given the test food to consume. Further blood samples will be taken at periodic intervals for the subsequent 3.5 hours. Blood samples will be taken every 15 minutes in the first 60 minutes and every 30 minutes for the remaining 150 minutes. At every time point blood samples will be extracted from a finger prick (for measuring glucose) and from the cannula (for measuring all other metabolites). At the same time points subjects will have to fill in visual analogue scales on feelings of hunger and satiety.

研究设计

研究类型
Interventional
分配方式
Randomized
干预模型
Crossover
主要目的
Other
盲法
None

入排标准

年龄范围
21 Years 至 40 Years(Adult)
性别
Male
接受健康志愿者

入选标准

  • Chinese ethnicity
  • Age between 21-40 years
  • Do not have any metabolic diseases (diabetes, hypertension etc)
  • Do not have G6PD deficiency
  • Not on prescription medication known to affect glucose related metabolism
  • Not allergic/intolerant to any of the test foods
  • Do not partake in sports at the competitive and/or endurance levels
  • Weight of at least 45kg
  • Body mass index between 18.0 to 24.9 kg/m2
  • Normal blood pressure (120/80 mmHg)
  • Fasting blood glucose <6.0 mmol/L

排除标准

  • 未提供

研究组 & 干预措施

Rice

Other

Control session - white rice (equivalent to 50g available carbohydrates)

干预措施: White rice (Other)

Rice with chicken

Active Comparator

Treatment 1 - White rice and steamed chicken breast (25g protein)

干预措施: Rice with chicken breast (Other)

Rice with fish

Active Comparator

Treatment 2 - White rice and steamed fish (25g protein)

干预措施: Rice with fish (Other)

Rice with egg white

Active Comparator

Treatment 3 - White rice and egg white (25g protein)

干预措施: Rice with egg white (Other)

Rice with beancurd

Active Comparator

Treatment 4 - White rice and steamed beancurd (25g protein)

干预措施: Rice with beancurd (Other)

结局指标

主要结局

Blood glucose response (incremental area under the curve) measured using the hemocue

时间窗: 3.5 hours post consumption

Blood glucose response (incremental area under the curve) measured using the hemocue

Glucagon determined using the ELISA

时间窗: 3.5 hours post consumption

Glucagon is determined using the ELISA

Insulin (incremental area under the curve) is determined using the COBAS

时间窗: 3.5 hours post consumption

Insulin (incremental area under the curve) is determined using the COBAS

次要结局

  • Effects of physical activity(Assessed at the start of every test session 1-2 weekly and up to 8 weeks in total per subject.)
  • Satiety: Hunger and fullness measured using the Visual analogue scale(Assessed at the start of every test session 1-2 weekly and up to 8 weeks in total per subject.)

研究者

发起方
Clinical Nutrition Research Centre, Singapore
申办方类型
Other Gov
责任方
Principal Investigator
主要研究者

Melvin Leow

Principal Investigator

Clinical Nutrition Research Centre, Singapore

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