Development of a New Diagnostic Tool to Assess Oocyte Quality Based on Tachykinin and Kisspeptin Expression Analysis in Human Granulosa and Cumulus Cells
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 236
- 试验地点
- 4
- 主要终点
- Expression level of neurokinin A (NKA)
研究概览
简要总结
The purpose of this project is to study the presence of expression differences - at RNA and protein level - of different members of the tachykinin family and kisspeptin and their receptors, between fertile women and infertile patients with different etiologies.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Two groups of subjects (each one with different criteria):
- •Oocyte donors
- •Inclusion Criteria:
- •Age between 18 and 34 years
- •Normal caryotype
- •Normal psychological test
排除标准
- •Presence of hereditary diseases
- •Beta-thalassemia
- •Cystic fibrosis
- •Human immunodeficiency virus (HIV), Hepatitis B virus (HBV), Hepatitis C virus (HCV)
- •Infertility patients
- •Inclusion Criteria:
- •Age between 18 and 45 years
- •One of the following etiologies:
- •Patient with PCOS according to Rotterdam Criteria or
- •Patients with endometriosis stage I-IV or
- •Patients with low ovarian response according to Bologna criteria or
- •Patients with advances maternal age (between 38 and 45 years)
结局指标
主要结局
Expression level of neurokinin A (NKA)
时间窗: 4 years
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Expression level of hemokinin 1 (HK-1)
时间窗: 4 years
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Expression level of kisspeptin 1 (KISS1)
时间窗: 4 years
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Expression level of Substance P (SP)
时间窗: 4 years
Expression analysis at mRNA (mRNA = messenger ribonucleic acid) and protein level using techniques of real time quantitative PCR (PCR = polymerase chain reaction), immunocytochemistry and western blot
Expression level of neurokinin B (NKB)
时间窗: 4 years
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Expression level of neurokinin 1 receptor (NK1R)
时间窗: 4 years
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Expression level of kisspeptin 1 receptor (KISS1R)
时间窗: 4 years
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Expression of neurokinin 2 receptor (NK2R)
时间窗: 4 years
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
Expression level of neurokinin 3 receptor (NK3R)
时间窗: 4 years
Expression analysis at mRNA and protein level using techniques of real time quantitative PCR, immunocytochemistry and western blot
次要结局
- Years of infertility(4 years)
- Fertilization rate(4 years)
- Embryo quality(4 years)
- Daily and total dose of FSH (FSH = follicle stimulating hormone)(4 years)
- Patient or donor age(4 years)
- Estradiol level (day of ovulation induction)(4 years)
- Patient or donor body mass index(4 years)
- Progesterone level (day of ovulation induction)(4 years)
- Number of oocytes retrieved in the ovarian puncture(4 years)
- Daily and total dose of HMG (HMG = human menopausal gonadotropin)(4 years)
- Number of mature oocytes (metaphase II) obtained in the ovarian puncture(4 years)
- Type of ovulation induction(4 years)
研究者
Manuel Fernandez-Sanchez
Dr
IVI Sevilla