The Relationship Between MMP-2 and TIMP-2 Gene Polymorphisms and Skin Barrier Function, Inflammatory Cytokine Levels in Acne Patients
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 300
- 试验地点
- 1
- 主要终点
- Genotype Frequencies of MMP-2 and TIMP-2
研究概览
简要总结
This study investigated the relationship between Matrix Metalloproteinase-2 (MMP-2) and Tissue Inhibitor of Metalloproteinase-2 (TIMP-2) gene polymorphisms and acne pathogenesis. The study aimed to determine whether specific genotypes (MMP-2-CC and TIMP-2-CC) are associated with impaired skin barrier function (measured by transepidermal water loss and skin hydration) and elevated levels of inflammatory cytokines (IL-1β, TNF-α) in acne patients compared to healthy controls.
详细描述
Acne vulgaris is a common dermatological condition with a complex pathogenesis involving skin barrier dysfunction and inflammation. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are crucial in extracellular matrix remodeling and have been implicated in acne. This case-control study was designed to explore the association of specific polymorphisms in the MMP-2 (rs243865) and TIMP-2 (rs8179090) genes with clinical and biological markers in acne. A total of 200 acne patients and 100 healthy controls were enrolled. Genomic DNA was extracted from peripheral blood samples, and genotyping was performed using PCR-RFLP. Skin barrier function was assessed by measuring Transepidermal Water Loss (TEWL) and skin hydration. Serum levels of the inflammatory cytokines Interleukin-1β (IL-1β) and Tumor Necrosis Factor-α (TNF-α) were quantified using ELISA. The study evaluates whether the CC genotypes of MMP-2 and TIMP-2 are risk factors for acne susceptibility and are correlated with more severe disease phenotypes, characterized by poorer skin barrier integrity and a heightened inflammatory state.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Crossover
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Aged 18 years or older, regardless of gender.
- •Diagnosed with acne by a dermatologist (for patient group).
- •No systemic or skin diseases (for control group).
- •Able to provide informed consent and willing to undergo genotype testing and related skin function tests.
排除标准
- •Acne induced by medications.
- •Polycystic ovary syndrome or other forms of hormone-related acne.
- •Any local, systemic, or surgical acne/acne scar treatments within the last four weeks.
- •Presence of genetic connective tissue diseases.
- •Associated with pyoderma or other systemic comorbidities.
- •Refusal to sign the informed consent form.
结局指标
主要结局
Genotype Frequencies of MMP-2 and TIMP-2
时间窗: Data for each participant were collected at a single visit during the study period from March 2018 to September 2021.
Comparison of the genotype distribution (CC vs. CT/TT for MMP-2; CC vs. GC/GG for TIMP-2) between acne patients and healthy controls to assess association with acne susceptibility. \[Time Frame: Data collected at a single study visit\] Skin Barrier Function Assessment: Measurement of Transepidermal Water Loss (TEWL) and skin hydration levels in acne patients, compared across different MMP-2 and TIMP-2 genotypes.
Skin Barrier Function Assessment
时间窗: Data for each participant were collected at a single visit during the study period from March 2018 to September 2021.
Measurement of Transepidermal Water Loss (TEWL) and skin hydration levels in acne patients, compared across different MMP-2 and TIMP-2 genotypes.
Serum Interleukin-1β (IL-1β) Level
时间窗: Data for each participant were collected at a single visit during the study period from March 2018 to September 2021.
Measurement of serum levels of IL-1β in acne patients, compared across different MMP-2 and TIMP-2 genotypes.
Serum Tumor Necrosis Factor-α (TNF-α) Level
时间窗: Data for each participant were collected at a single visit during the study period from March 2018 to September 2021.
Measurement of serum levels of TNF-α in acne patients, compared across different MMP-2 and TIMP-2 genotypes.
次要结局
- Correlation Analysis(Analysis was performed after the completion of data collection in September 2021.)
研究者
Hong Zhang
Principal investigator
Shijiazhuang TCM Hospital