Effect of Non-surgical Periodontal Treatment on Visfatin, fetuin-a and Sirtuin 1 Concentrations in Gingival Crevicular Fluid of Patients With Periodontitis and Type 2 Diabetes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 66
- 试验地点
- 2
- 主要终点
- Bleeding on probing index (BPI)
研究概览
简要总结
The aim of this study is to determine the concentrations of Visfatin, Fetuin-A and Sirtuin 1 in the gingival crevicular fluid and clinical periodontal parameters in diabetic and systemically healthy individuals and to determine whether non-surgical periodontal treatment had any effect on these biomarkers and periodontal clinical parameters at the end of a 3-month follow-up period. The hypothesis of our study is that gingival crevicular fluid Visfatin, Fetuin-A and Sirtuin 1 concentrations will change with non-surgical periodontal treatment in type 2 diabetic and systemically healthy individuals and that this change will be associated with diabetes and clinical parameters.
详细描述
Sixty-six patients were divided into six equal groups; group 1: systemically and periodontally healthy individuals, group 2: systemically healthy individuals with periodontitis, group 3: controlled type 2 diabetes and periodontally healthy individuals, group 4: controlled type 2 diabetes and periodontitis, group 5: uncontrolled type 2 diabetes and periodontally healthy individuals, group 6: uncontrolled type 2 diabetes and periodontitis. The study was planned as a randomized, single-blind, parallel design. Periodontal clinical parameters (Silness-Löe plaque index, Löe-Silness gingival index, probing pocket depth, clinical attachment level, bleeding on probing) were recorded clinically and concentrations of visfatin, fetuin-A and sirtuin 1 in gingival crevicular fluid were assessed biochemically. After baseline examinations, periodontal treatment was completed using a nonsurgical periodontal treatment protocol completed in 24 hours. Periodontal clinical parameters and gingival crevicular fluid were recorded at baseline and 3 months after periodontal treatment. Biochemical analysis and statistical evaluation were performed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 31 Years 至 81 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Volunteering to participate in the study
- •To be over 18 years old
- •No periodontal treatment in the last 6 months
- •Being systemically healthy except for type 2 diabetes
- •Not taking medication for any reason except type 2 diabetes
- •HbA1c <7 in controlled Type 2 diabetes group
- •HbA1c ≥7 in uncontrolled Type 2 diabetes group
- •Not smoking or drinking alcohol
排除标准
- •Not volunteering to participate in the study
- •Under 18 years of age
- •Periodontal treatment in the last 6 months
- •Having any systemic disease affecting the periodontal condition
- •Having used local or systemic antibiotics in the last 3 months
- •Use of anti-inflammatory, steroid drugs in the last 3 months
- •Taking vitamin, mineral or antioxidant supplements in the last 3 months
- •Being pregnant or lactating
- •Regular use of mouthwash
- •Smoking or drinking alcohol
结局指标
主要结局
Bleeding on probing index (BPI)
时间窗: Baseline to 3 months after treatment
BPI was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on BPI in periodontitis patients. BPI was reduced after NSPT.
Visfatin concentration in GCF
时间窗: Baseline to 3 months after treatment
Visfatin concentrations in GCF were increased in all periodontitis patients. In periodontal healthy patients with Type 2 diabetes visfatin concentrations in GCF were increased. Non-surgical periodontal treatment decreased the concentration of visfatin in GCF.
Gingival crevicular fluid (GCF) quantity
时间窗: Baseline to 3 months after treatment
The amount of gingival crevicular fluid was not affected by periodontal status in patients with Type 2 diabetes.
Plaque index (PI)
时间窗: Baseline to 3 months after treatment
PI was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on PI in periodontitis patients. PI was reduced after non-surgical periodontal treatment (NSPT).
Clinical attachment level (CAL)
时间窗: Baseline to 3 months after treatment
CAL was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on CAL in periodontitis patients. CAL was reduced after NSPT.
Gingival index (GI)
时间窗: Baseline to 3 months after treatment
GI was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on GI in periodontitis patients. GI was reduced after NSPT.
HbA1c level in blood plasma
时间窗: Baseline to 3 months after treatment
NSPT reduced HbA1c levels of patients with type 2 diabetes.
Sirtuin 1 concentration in GCF
时间窗: Baseline to 3 months after treatment
In systemic healthy individuals, periodontitis decreased the concentration of sirtuin 1 in GCF. Type 2 diabetes increased sirtuin 1 concentration in GCF. In controlled type 2 diabetes patients with periodontitis sirtuin 1 concentrations in GCF were increased. In uncontrolled type 2 diabetes patients with periodontitis sirtuin 1 concentrations in GCF were decreased. Non-surgical periodontal treatment increased the concentration of sirtuin 1 in GCF.
Probable pocket depth (PPD)
时间窗: Baseline to 3 months after treatment
PPD was increased in all periodontitis patients with or without type 2 diabetes. Type 2 diabetes has no effect on PPD in periodontitis patients. PPD was reduced after NSPT.
Fetuin-A concentration in GCF
时间窗: Baseline to 3 months after treatment
Fetuin-A concentrations in GCF were decreased in all periodontitis patients. In periodontal healthy patients with Type 2 diabetes fetuin-A concentrations in GCF were increased. Non-surgical periodontal treatment increased the concentration of fetuin-A in GCF.
次要结局
未报告次要终点
研究者
Burcu Ozkan Cetinkaya
Professor in Periodontology
Ondokuz Mayıs University