Analysis of B Cells From Autoimmune Individuals
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 46
- 试验地点
- 1
- 主要终点
- Frequency of CD19 and IgM low B cells in cord blood of babies born from autoimmune mothers
研究概览
简要总结
This observational study aims at finding out if individual with autoimmunity exhibit increased numbers of B cells that express two types (instead of one type) of antibodies, and if B cells of individuals genetically susceptible to autoimmunity display defects in the biological process of tolerance, which removes B cells that participate in autoimmunity.
详细描述
Peripheral blood B cells of SLE patients are analyzed by flow cytometry to determine the frequency of B cells co-expressing immunoglobulin kappa an lambda light chains. This frequency is correlated to the disease activity.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Adult Males and Females diagnosed with Lupus;
- •Adult Females who are pregnant and diagnosed with:
- •lupus erythematosus
- •rheumatoid arthritis, or
- •type 1 diabetes.
排除标准
- •Adult males or adult females treated with B-cell depletion therapies.
结局指标
主要结局
Frequency of CD19 and IgM low B cells in cord blood of babies born from autoimmune mothers
时间窗: 1 day
For cord blood samples, the main outcome measurement is the frequency of B cells that express both low levels of CD19 and IgM within the total (CD19+ or CD20+) B cell population. The collected data will be aggregated as the average frequency of CD19low/IgMlow B cells in subjects that were born from autoimmune mothers relative to those born from healthy mothers. Moreover, data will also be stratified in respect to the presence or absence of the PTPN22-R620W allele, which is a risk allele for autoimmunity.
Frequency of kappa to lambda ratios of B cells in blood of autoimmune adults
时间窗: 1 day
For peripheral adult blood, the main outcome measure is the frequency of κ/λ (kappa to lambda ratios) dual immunoglobulin B cells within the overall B (CD19+ or CD20+) cell population. The collected data will be aggregated as the average frequency of κ/λ B cells in subjects with autoimmunity relative to healthy controls.
次要结局
未报告次要终点