Consecutive, Case Control Study of Association Between Single Nucleotide Polymorphism of SP-D and Susceptibility and Prognosis of Infectious Keratitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 145
- 试验地点
- 1
- 主要终点
- Genotype
研究概览
简要总结
This study explores the association between single nucleotide polymorphism (Met11Thr) of surfactant protein D(SP-D) and susceptibility and prognosis of infectious keratitis.Met11Thr of SP-D of patients with keratitis and normal controls were compared.Allele and genotype frequencies of patients with keratitis and normal controls were determined by polymerase chain reaction with sequence specific primers(PCR-SSPs) .SP-D gene polymorphism of patients with keratitis and normal controls was detected by Sanger sequencing
详细描述
Surfactant protein D(SP-D) plays an important role in the innate immune system of the eye in the lacrimal duct, cornea and conjunctiva.The single lipopolysaccharide on the surface of bacteria and the receptors on the surface of immune cells are linked by SP-D ,which acts as a bridge to neutralize viruses in the innate immune defense system and assists other immune cells in clearing bacteria and fungi.SP-D can also participate in the acquired immune response after combining with lipopolysaccharide on the surface of microorganisms.Some single nucleotide polymorphisms of SP - D can not only affect the plasma level of SP - D, which leads to the differences of pathogen defense capability, but also can affect the space configuration and structure of carbohydrate recognition domain of SP-D, causing the change of ability of SP - D combined with pathogens to influence its defensive role.The concentration of SP - D in ocular surface is closely related to the occurrence and progression of infectious keratitis.Association between single nucleotide polymorphisms of SP-D and the occurrence and development of infectious keratitis has yet to be confirmed by research.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Clinical diagnosis of Infectious Keratitis.
- •Laboratory diagnosis of Infectious Keratitis.
排除标准
- •Patients with topical or systemic long-term use of hormones, interferon, or immunosuppressive therapy.
- •Patients with basic diseases such as diabetes, autoimmune disease, sjogren's syndrome, blepharitis, eyelid defect or incomplete eyelid closure.
- •Patients with history of corneal myopia laser, cataract surgery and vitrectomy, or history of serious ocular trauma and drug allergies.
研究组 & 干预措施
Control Group
health volunteers
干预措施: Sequencing (Other)
Infectious Keratitis Group
Infectious corneal patients
干预措施: Treatment of bacterial keratitis (Drug)
Infectious Keratitis Group
Infectious corneal patients
干预措施: Treatment of fungal keratitis (Drug)
Infectious Keratitis Group
Infectious corneal patients
干预措施: Treatment of viral keratitis (Drug)
Infectious Keratitis Group
Infectious corneal patients
干预措施: corneal scraping (Diagnostic Test)
Infectious Keratitis Group
Infectious corneal patients
干预措施: Sequencing (Other)
结局指标
主要结局
Genotype
时间窗: before the treatment
Genotype of SNP（rs721917）:( Met /Met , Thr/Thr and Met/Thr)
Base type
时间窗: before the treatment
Base type of SNP（rs721917）:(Met and Thr)
次要结局
- Grading of Keratitis(before the treatment)