Alternative Splicing and Leukemia Initiating Cells
试验速览
- 阶段
- 不适用
- 入组人数
- 500
- 试验地点
- 1
- 主要终点
- Polymerase chain reaction
研究概览
简要总结
Aberrant RNA splicing and mutations in spliceosome complex in acute myeloid leukaemia (AML) are frequent. It have been shown that some splicing variants had a prognostic value in AML.
AML are characterized by their propensity to relapse because of the persistence of leukaemia initiating cells (LICs).
The aim of this study is to determine the splice variants on AML initiator cells and define a splicing pattern.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Age ≥ 18 years-old
- •Patients treated at the south lyon hospital center
- •Patients with a diagnosis of acute myeloid leukemia confirmed in cytology and whose involvement of the 11q23 locus was confirmed by in situ hybridization
- •Patients for whom a sample is available in the cytogenetic laboratory of the south lyon hospital center
排除标准
- 未提供
结局指标
主要结局
Polymerase chain reaction
时间窗: 1 month
Transcriptome analysis to determine the splicing variants on acute myeloid leukaemia initiator cells. The analysis will focus on the gene encoding ABCA3 transporter and genes known to be mutated in patients with acute myeloid leukemia (FLT3, NPM1, c-Kit, N et K-RAS). The duration of selection of samples is estimated to be one month.
Ribonucleic acid sequencing
时间窗: 1 month
Transcriptome analysis to determine the splicing variants on acute myeloid leukaemia initiator cells. The analysis will focus on the gene encoding ABCA3 transporter and genes known to be mutated in patients with acute myeloid leukemia (FLT3, NPM1, c-Kit, N et K-RAS). The duration of selection of samples is estimated to be one month.
次要结局
未报告次要终点