Establishing Continuous Cell Lines and Xenografts From Pediatric Cancers for Biological and Pre-Clinical Therapeutic Studies
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 213
- 试验地点
- 68
- 主要终点
- Creation of a bank of cell lines and generation of sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols
研究概览
简要总结
This laboratory study is collecting and storing tissue, blood, and bone marrow samples from young patients with cancer. Collecting and storing samples of tissue, blood, and bone marrow from patients with cancer to study in the laboratory may help doctors learn more about changes that may occur in DNA and identify biomarkers related to cancer.
详细描述
PRIMARY OBJECTIVES:
I. Establish and bank cell lines and/or xenografts from pediatric patients with cancer.
II. Establish continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer.
III. Establish transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro.
IV. Create a bank of cell lines and generate sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols. V. Characterize cell lines from childhood cancers with respect to DNA short tandem repeat molecular profile as a "fingerprint" of original cell line identity.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- — 至 21 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •All malignant tissues from childhood cancers allowed including the following:
- •Brain tumors (all types)
- •Tissue should be submitted to CNS Committee Resource labs to be forwarded for this study, unless instructed otherwise on the COG web site
- •Ewing family of tumors
- •Rhabdomyosarcomas
- •Other soft tissue sarcomas
- •Osteogenic sarcomas
- •Rhabdoid tumors
- •Neuroblastomas
- •Viable material for cell culture for neuroblastoma is collected via COG-ANBL00B1 and should not be submitted via this study unless the patient cannot be enrolled on COG-ANBL00B1*
- •Retinoblastomas
- •Anaplastic Wilms tumor
- •Germ cell tumors
- •Leukemias/lymphomas
- •Acute myeloid leukemia (AML)
- •Blood samples and bone marrow samples from patients at second relapse and beyond may be submitted for this study
- •Bone marrow samples at diagnosis or first relapse must be submitted to an AML resource lab and will be forwarded for this study at the discretion of the AML Committee
- •Acute lymphoblastic leukemia (ALL)
- •Blood samples may be submitted directly to this study
- •Bone marrow samples must be submitted to an ALL resource lab and will be forwarded for this study at the discretion of the ALL Committee
- •Enrolled on a COG therapeutic, biology, or tissue banking protocol that allows collection of tissue for research and submission to a COG-designated resource laboratory
- •Participation in this protocol is not permitted until after tissue requirements for any active COG disease-specific therapeutic, biology, or banking protocols have been satisfied
- •Material may only be submitted for this protocol if tissue is available in excess of that required for satisfying active disease-specific therapeutic and biological protocols
- •Patients with diagnosis pending are eligible
排除标准
- 未提供
结局指标
主要结局
Creation of a bank of cell lines and generation of sufficient vials of cryopreserved cells for distribution to investigators with approved COG biology protocols
时间窗: Up to 14 years
Characterization of cell lines for expression of molecular makers that confirm the tumor-type of the cell line and the immortal nature of the cells (telomerase) and the expression of molecular markers that may correlate with drug resistance
时间窗: Up to 14 years
Establishment of continuous cell lines, under carefully controlled conditions, from pediatric patients with cancer
时间窗: Up to 14 years
Characterization of cell lines for the ability for sustained growth in tissue culture and/or as mouse xenografts
时间窗: Up to 14 years
Characterization of cell lines for mycoplasma contamination
时间窗: Up to 14 years
Establishment and banking of cell lines and/or xenografts from pediatric patients with cancer
时间窗: Up to 14 years
Establishment of transplantable xenografts in immunocompromised mice from tumor cells that are difficult to establish as continuous cell lines in vitro
时间窗: Up to 14 years
Characterization of cell lines from childhood cancers with respect to DNA PCR molecular HLA profile as a "fingerprint" of original cell line identity
时间窗: Up to 14 years
次要结局
未报告次要终点