Profiles Analysis of Genome Wide Association Study (GWAS) in Acne Vulgaris in Indonesia
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- Population structure
研究概览
简要总结
This study is a series of descriptive-analytic studies on genomic variants in the facial skin of patients with AV at the Dermatology and Venereology Polyclinic by determining the genetic profile by GWAS examination in AVB and analysing the genetic profile in AVB against differences in geographical location. The research design used was a cross-sectional design.
详细描述
Research subjects were obtained from the affordable population who met the research criteria and expressed their willingness to participate in the study.
Subject selection was carried out by purposive sampling method that met the inclusion criteria. The study will be conducted in one visit where the patient and his/her parents come to be given an explanation about the study with a request for informed consent, screening for inclusion and exclusion criteria, anamnesis of facial complaints, taking data on demographics, patient's medical history, patient's medical history, physical examination, taking clinical photos in 5 positions and ending with taking blood samples of the patient and blood samples from both parents of the patient as much as 10 mL.
The study will be conducted in three places, namely in Jakarta, Padang, and Manado. In sampling in Padang and Manado, to prevent damage to the samples that have been taken, the samples will be stored at a temperature of -20C, then when brought to Jakarta will use a cooler box along with an ice pack at a temperature of 2-3C, before being processed in the Bioinformatics Core Facilities IMERI laboratory, Jakarta. When the samples are collected in Jakarta, the blood samples will be immediately stored in the laboratory at -20C.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 17 Years 至 60 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Males or females with AVB aged 17-21 years
- •Both parents of complete subjects with or without a history of acne vulgaris aged up to 60 years
- •Patients and parents are willing to participate in the study and sign informed consent.
排除标准
- •Patients who have other inflammatory or infectious diseases especially on the face
- •Have genetic diseases or disorders
- •Diagnosed with primary or secondary immunodeficiency diseases
- •Using oral or systemic antibiotic, anti-inflammatory, retinoid or hormone therapy in the past month.
结局指标
主要结局
Population structure
时间窗: 3 months
Gene testing of blood sample Genetic Inflation Factor (GIF) \>1.1 or \<1.1 Principal Component Analysis (PCA)
Severity of acne vulgaris
时间窗: 1 year
Physical examination: identify the number of blackheads, papules, pustules and cysts. Degree of severity:(1)Mild =Comedo\<20, or inflammatory lesion\<15, or total lesion\<30(2)Moderate = Comedo 20-100, or inflammatory lesion15-50, or total lesion 30-125(3)Severe =cyst\>5, or Comedo\>100, or inflammatory lesion\>50, or total lesion\>125.
Genomic Profile
时间窗: 3 months
Blood sample examination, processed using GWAS Mutations (SNPs)(1)Present(2)Absent
SNP Enrichment
时间窗: 3 months
Gene testing of blood samples Mutation position
Association analysis and Association analysis of imputation results
时间窗: 3 months
Gene testing of blood samples PCA value
Linkage disequilibirium (LD)
时间窗: 3 months
Gene testing of blood samples Coefficient 0-1
Haplotype
时间窗: 3 months
Gene testing of blood samples Jumlah gen 1. Satu gen 2. Multipel gen
次要结局
未报告次要终点
研究者
Dr.dr.Irma Bernadette, SpKK (K)
Prof. Dr. dr. Irma Bernadette S. Sitohang, Sp.KK (K)
Indonesia University