Nutrient and Hormonal Profile and Muscle Protein Synthesis Response to Consuming Chicken Grown Using Different Farming Practices
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 入组人数
- 19
- 试验地点
- 2
- 主要终点
- post-prandial amino acid profile
研究概览
简要总结
The study aims to evaluate if there is a difference between eating conventionally raised or organic chicken. The investigators will measure and compare the nutrient and hormone levels in the participant's blood and the muscle protein synthesis rate (the rate at which your body builds muscle) after eating chicken from different farming practices. This will help the investigators to understand if these farming practices impact muscle protein synthesis and overall health.
Specific aim 1: Describe the post-prandial nutrient and hormonal profile in serum in the 3 hours following consumption of 100 grams (~32g of protein) of boneless-skinless conventional vs. regenerative chicken breast meat.
Specific aim 2: Compare the ability of boneless-skinless chicken breasts grown with these two farming practices to activate mTORC1-specific and whole muscle protein synthesis in an in vitro model of muscle.
详细描述
Indicators of longevity in humans include skeletal muscle mass and strength. Muscle mass depends on the balance between myofibrillar protein synthesis and degradation, influenced by diet and physical activity. Muscle strength correlates with muscle mass, and weight lifting until failure increases muscle protein synthesis, enhancing muscle mass and strength over time. Protein intake is crucial for positive protein balance and muscle growth. Animal proteins promote muscle synthesis more effectively than plant proteins. Limited research exists on the impact of agricultural practices on meat quality. Studies indicate that organic meat may have higher nutritional value and better lipid profiles. This project aims to compare the serum nutrient, hormonal profiles, and muscle protein synthesis rates after consuming isonitrogenous amounts of chicken from conventional versus regenerative/organic practices.
The purpose of the study is to quantify and compare the serum nutrient and hormonal profile, and muscle protein synthesis rates, in response to consuming isonitrogenous amounts of chicken grown using different agricultural practices (conventional vs. regenerative)
Specific aim 1: Describe the post-prandial nutrient and hormonal profile in serum in the 3 hours following consumption of 100 grams (~32g of protein) of boneless-skinless conventional vs. regenerative chicken breast meat.
Specific aim 2: Compare the ability of boneless-skinless chicken breasts grown with these two farming practices to activate mTORC1-specific and whole muscle protein synthesis in an in vitro model of muscle.
Study participant will come to the CTSC Clinical Research Center on two separate occasions.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Other
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
盲法说明
A randomized double-blind crossover design with neither the subjects nor the investigators knowing who is on which food. The interventions will be coded using a blinded alphabetical letter code (A, B). A delegate researcher (independent party not further involved in the study) will randomize all interventions using a computer-generated randomization list. The delegate will hand in the code-breaker to the principal investigator in a sealed envelope. The envelope will be stored in a locked filing cabinet in the principal investigator's office, which will also be locked when unoccupied. The envelope will be opened by the principal investigator after completing the analysis of blood samples.
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy active males and females (To be considered active, volunteers must meet the following American College of Sports Medicine's guideline for physical activity for healthy adults: performing at least 150 minutes per week of moderate-to-vigorous intensity physical activity.
- •Age18-30 years
- •Normal weight (BMI between 18 and 25 kg/m2
排除标准
- •Health or dietary restrictions that would prevent consumption of the test foods
- •Known food allergy to chicken
- •Anemia (low red blood cell count)
- •Overweight or obesity (BMI > 25 kg/m2)
- •Receiving any medication that may interfere with the study
- •Metabolic or endocrine disorder that would affect the digestion, absorption, and/or physiological response to any of the nutrients ingested.
- •Currently not meeting the ACSM physical activity recommendations (IPAQ score < 150 min/week of moderate-to-vigorous physical activity.
研究组 & 干预措施
Regenerative Chicken
This arm will be given a meal of 100 grams (~32g of protein) boneless-skinless regenerative chicken breast meat together with 250mL of water
干预措施: Regenerative Chicken (Other)
Conventional Chicken
This arm will be given a meal of 100 grams (~32g of protein) boneless-skinless conventional chicken breast meat together with 250mL of water
干预措施: Conventional Chicken (Other)
结局指标
主要结局
post-prandial amino acid profile
时间窗: Baseline (0 hour) to 30, 60, 90, 120 and 180 minutes after consumed meal
Serum samples are extracted with 1mL of modified Folch extraction, followed by vacuum centrifugation for drying. Dried samples will be reconstituted with 100 μl of 75% ACN/water. The supernatant is then injected into Aligent UPLC-QqQ for analysis of targeted free amino acids, short peptides, and other metabolites.
Muscle protein synthesis
时间窗: Baseline (0 hour) to 30, 60, 90, 120 and 180 minutes after consumed meal
To measure muscle protein synthesis, stably transfected C2C12 muscle cells with a plasmid (pcDNA3 luciferase) will be used. C2C12Luc cells will be plated in 24-well plates and differentiated over 4 days. Differentiated C2C12 cells will be fasted by washing with PBS and then treating them with Test Media (20% DMEM) for 15 minutes. Fasted muscle cells will then be treated with Test Media containing 10% baseline or fed serum (from blood samples at 30, 60, 90,120 and 180 minutes after meal) for 3 hours. Cells will be collected in passive lysis buffer and firefly luciferase activity will be determined.
mTORC1 specific protein synthesis
时间窗: Baseline (0 hour) to 30, 60, 90, 120 and 180 minutes after consumed meal
To measure the ability of meal of chicken (conventional or regenerative) to activate mTORC1, stably transfected C2C12 muscle cells with a plasmid (pcDNA3-TOP luciferase) where the luciferase mRNA contains a 5'TOP. 5'TOP mRNA, which specifically regulated by mTORC1 activity, will be used. Differentiated C2C12TOPLuc muscle cells in 24-well plates will be stimulated using the baseline or fed serum (from blood samples at 30, 60, 90,120 and 180 minutes after meal). The degree of mTORC1 activation will be determined as the difference in slopes between the baseline and fed sera.
post-prandial short peptide profile
时间窗: Baseline (0 hour) to 30, 60, 90, 120 and 180 minutes after consumed meal
Serum samples are extracted with 1mL of modified Folch extraction, followed by vacuum centrifugation for drying. Dried samples will be reconstituted with 100 μl of 75% ACN/water. The supernatant is then injected into Aligent UPLC-QqQ for analysis of targeted free amino acids, short peptides, and other metabolites.
次要结局
未报告次要终点