Postprandial Monocyte Maturation and Vascular Dysfunction Following High-Fat Meals: The Impact of Cardiovascular Fitness and Acute Aerobic Exercise - Study 1
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 59
- 试验地点
- 1
- 主要终点
- Fasting LDL/HDL ratio
研究概览
简要总结
The purpose of this research study is to examine the effect of high-fat meals on the health of blood vessels. In addition, the study will examine how exercise/fitness/physical activity impacts blood vessels after consumption of a high-fat meal.
详细描述
This is the first in a series of studies examining the impact of high-fat meals on blood vessels. During the preliminary visit of this study, eligible participants' resting metabolic rate, arm flow mediated dilation, and leg flow mediate dilation will be measured. They will complete handgrip and plantar flexion exercise tasks. Finally, they will be given a physical activity monitor to wear for 7 days. At their first visit, participants will eat a high-fat meal and blood will be drawn to measure blood vessel health. They will also repeat the arm flow mediated dilation, leg flow mediate dilation, handgrip, and plantar flexion exercise tests.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •college-age: 18-30 years old
- •normal fasting triglyceride (<150 mg/dL) (American College of Sports Medicine Guidelines for Exercise Testing and Prescription, 10th Edition).
- •normal body composition: % body fat, males <25%, females <32% (American College of Sports Medicine Guidelines for Exercise Testing and Prescription, 10th Edition)
- •self-reported engagement in moderate-vigorous intensity physical activity and/or an exercise training regimen OR self-reported low physical activity and no engagement in an exercise training regimen (International Physical Activity Questionnaire)
- •low cardiorespiratory fitness (VO2peak; male: =<45 mL/kg/min; female: =<35 mL/kg/min; categorized as =40th percentile by ACSM Guidelines for Exercise Testing and Prescription, 10th Edition; 44, 45, 46, 47) OR high cardiorespiratory fitness (VO2peak; male: =55 mL/kg/min; female: 45 mL/kg/min; categorized as =70th percentile by ACSM Guidelines for Exercise Testing and Prescription, 10th Edition; 44, 45, 46, 47)
- •f.) for female participants, presence of a normal, monthly menstrual cycle with or without prescribed contraceptive methods.
排除标准
- •presence of diagnosed cardiovascular, metabolic, or renal disease or dysfunction
- •presence of signs and symptoms suggestive of cardiovascular, metabolic, or renal disease
- •presence of musculoskeletal injury
- •history of smoking
- •engagement in an abnormal eating behavior
- •unable to communicate effectively in English
- •moderate cardiorespiratory fitness (VO2peak; male: 46-54 mL/kg/min; female: 36-44 mL/kg/min)
- •elevated or high fasting triglycerides (>150 mg/dL)
- •absence of a normal, monthly menstrual cycle with or without prescribed contraceptive methods
研究组 & 干预措施
Low physical activity/fitness
Participants will be classified as into low-physical fitness based on self-reported physical activity and cardiorespiratory fitness testing.
干预措施: High-fat meal (Other)
High physical activity/fitness
Participants will be classified as into high-physical fitness based on self-reported physical activity and cardiorespiratory fitness testing.
干预措施: High-fat meal (Other)
结局指标
主要结局
Fasting LDL/HDL ratio
时间窗: Baseline
Assessment of risk for heart disease.
Post HFM superficial artery flow-mediated dilation
时间窗: 5.5 hours
Dilation of superficial artery when blood flow increases in artery. Measured by non-invasive ultrasound.
Fasting superficial artery flow-mediated dilation
时间窗: Baseline
Dilation of superficial artery when blood flow increases in artery. Measured by non-invasive ultrasound.
Post HFM total cholesterol
时间窗: 4 hours
Type of lipid that is required for cell structure, but can contribute to increased risk of heart disease if high due to development of fatty deposits on vascular wall.
Post HFM HDL
时间窗: 4 hours
High density lipoprotein that transports fat molecules in the body. Associated with carrying cholesterol to the liver for degradation.
Post HFM LDL/HDL ratio
时间窗: 4 hours
Assessment of risk for heart disease.
Post HFM Triglycerides
时间窗: 4 hours
Ester derived from glycerol and three fatty acids, representative of the main constituent of body fat in humans.
Fasting HDL
时间窗: Baseline
High density lipoprotein that transports fat molecules in the body. Associated with carrying cholesterol to the liver for degradation.
Fasting non-HDL
时间窗: Baseline
Total cholesterol minus HDL, often associated with a better assessment of risk for heart disease.
Fasting anti-inflammatory surface receptor expression
时间窗: Baseline
CD14, CD206 - monocyte receptors associated with defining subsets and inflammatory differentiation of macrophages.
Post HFM anti-inflammatory surface receptor expression
时间窗: 4 hours
CD14, CD206 - monocyte receptors associated with defining subsets and inflammatory differentiation of macrophages.
Fasting pro-inflammatory surface receptor expression
时间窗: Baseline
CD16, CD86 - monocyte receptors associated with defining subsets and inflammatory differentiation of macrophages.
Post HFM brachial artery flow-mediated dilation
时间窗: 5.5 hours
Dilation of brachial artery when blood flow increases in artery. Measured by non-invasive ultrasound.
Fasting triglycerides
时间窗: Baseline
Ester derived from glycerol and three fatty acids, representative of the main constituent of body fat in humans.
Fasting total cholesterol
时间窗: Baseline
Type of lipid that is required for cell structure, but can contribute to increased risk of heart disease if high due to development of fatty deposits on vascular wall.
Fasting LDL
时间窗: Baseline
Low density lipoprotein that transports fat molecules in the body. Associated with high levels of cholesterol.
Post HFM LDL
时间窗: 4 hours
Low density lipoprotein that transports fat molecules in the body. Associated with high levels of cholesterol.
Post HFM non-HDL
时间窗: 4 hours
Total cholesterol minus HDL, often associated with a better assessment of risk for heart disease.
Post HFM pro-inflammatory surface receptor expression
时间窗: 4 hours
CD16, CD86 - monocyte receptors associated with defining subsets and inflammatory differentiation of macrophages.
Fasting brachial artery flow-mediated dilation
时间窗: Baseline
Dilation of brachial artery when blood flow increases in artery. Measured by non-invasive ultrasound.
次要结局
未报告次要终点