Biomarkers Expression in Gingival Crevicular Fluid and Accelerated Tooth Movement
试验速览
- 阶段
- 不适用
- 入组人数
- 25
- 主要终点
- Inflammatory Cytokines ad Chemokines Detection Assay
研究概览
简要总结
Gingival Crevicular Fluid samples will be collected using periopapers after accelerated tooth movement technique, results will be assessed with biomarkers levels done using ELISA Assays
详细描述
For this prospective study, 22 individuals were selected, with skeletal discrepancies randomly divided into two groups: 1 - accelerated tooth movement technique (ATMT) and 2 - Control.
Orthodontic records done in two times, initial and preoperative; Gingival Crevicular Fluid samples will be collected using periopapers in 7 times (1, 2, 7, 14, 28 days and after space closure- end of orthodontic treatment movement activation).
Flapless cortical perforations will be made in ATMT Group after dental extraction. Surgical protocol will be flapless to preserve periodontal health.
After extraction space closure, results will be assessed by clinical effects with biological markers levels:
CCL3/MIP-1 alpha, CCL5/RANTES, CXCL8/IL8, IL-1 alpha, IL-1 beta, IL6, TNF-alpha, TRANCE/RANK L, OCN OPG IL-10 VEGF TGF-ℬ
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Adult subjects ages 18-40 years old, in good general health, with adult complete dentition, regardless of presence of third molars.
- •Indication for orthognathic surgery
- •Tooth extraction indication for orthodontic treatment
- •Do not smoke
- •Periodontal and teeth healthy
- •Do not use bisphosphonates or corticosteroids
排除标准
- •Surgically assisted rapid palatal expansion indication
- •Cleft lip and palate
- •Craniofacial syndrome
- •Mental disorder
- •Metabolic diseases
研究组 & 干预措施
Control
Control group, with traditional orthodontic mechanics for anterior retraction. Crevicular fluid will be collected during anterior retraction mechanics to identified biomarkers in the inflammatory process
Acceleration
Group with micro-osteoperforation for accelerated tooth movement during anterior retraction, Crevicular fluid will be collected during anterior retraction mechanics to identified biomarkers in the inflammatory process
干预措施: micro-osteoperforation (Procedure)
结局指标
主要结局
Inflammatory Cytokines ad Chemokines Detection Assay
时间窗: 8 months
Measure the different levels of listed cytokines and chemokines after starting the anterior retraction
Bone remodeling markers in GCF samples
时间窗: 8 monnths
Measure the different levels of resorption biomarkers and bone formation after starting the anterior retraction
次要结局
未报告次要终点
研究者
LIANA FATTORI ABATI
MS, PhD
University of Sao Paulo