Study on the Relationship Between Neutrophil-mediated Thrombocytopenia and the Condition and the Course of Disease in Patients With HFRS
试验速览
- 阶段
- 不适用
- 入组人数
- 28
- 试验地点
- 1
- 主要终点
- Changes in the platelet activation ratio, %
研究概览
简要总结
Thrombocytopenia is an important pathological feature of HFRS（Hemorrhagic Fever With Renal Syndrome, HFRS）. However, the cause of thrombocytopenia in HFRS is not yet fully understood. Neutrophils, as the largest number of white blood cells in the human body, can widely participate in the immune process of viral infections. Previous studies have found that the neutrophils of patients with acute myocardial infarction can interact with activated platelets and further mediate platelet phagocytosis.Therefore, this study aims to systematically elucidate the immunological process of neutrophil mediated thrombocytopenia in patients with HFRS by exploring the correlation between platelet activation, neutrophil activation and neutrophil proportion of adherent / phagocytic platelets peripheral blood of HFRS patients, and to analyze its relationship with the course of HFRS disease, which lays a theoretical foundation for further understanding the pathogenesis of HFRS and provides a basis for clinical use.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Age ≥18 years;
- •Clinical diagnosis meets the diagnostic criteria of the national HFRS prevention and treatment plan (Hantavirus-specific IgM antibody is positive). The classification and staging standards of HFRS patients are based on the "Expert Consensus of Shaanxi Province on Diagnosis and Treatment of Hemorrhagic Fever with Renal Syndrome".
排除标准
- •Age <18 years;
- •Have a history of kidney disease;
- •Have a history of liver disease;
- •Have a history of malignant tumor;
- •Receive dialysis treatment before admission;
- •Combined with hypertension, coronary heart disease and diabetes history;
- •Combined with HIV infection and patients with autoimmune diseases and pregnancy.
结局指标
主要结局
Changes in the platelet activation ratio, %
时间窗: through study completion, an average of 2 year
flow cytometry
Changes in neutrophils MPO content, MFI
时间窗: through study completion, an average of 2 year
enzyme-linked immunosorbent assay
Changes in the neutrophils with internalized platelets, %
时间窗: through study completion, an average of 2 year
flow cytometry
Changes in the neutrophils with adherent platelets, %
时间窗: through study completion, an average of 2 year
flow cytometry
次要结局
- Changes in the platelets with phosphatidylserine exposure,%(through study completion, an average of 2 year)
- Changes in Neutrophils Mac-1 expression, MFI(through study completion, an average of 2 year)