Antibacterial Effect of the Infusion of Green Tea (Camellia Sinensis) as a Mouthwash on Saliva and Bacterial Plaque
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 28
- 试验地点
- 1
- 主要终点
- Antimicrobial effect
研究概览
简要总结
Given that the participation of oral microorganisms in the development of the most prevalent pathologies of the oral cavity is unquestionable, efforts have been made to create agents that allow controlling these bacteria. The objective of this work was to analyze the antibacterial effect of green tea infusion used as a mouthwash on saliva and bacterial plaque. The green tea infusion was prepared at 13% at approximately 90 ° C, being the same applied to 14 participants from the 1st to the 5th grade (experimental group); and to other 14 participants the placebo was applied (control group). The antibacterial effect was determined by counting the colony forming units (CFU) in the cultures of samples of bacterial plaque and saliva, taken before the application of the infusion and immediately after.
详细描述
Oral diseases, including dental caries, is one of the most prevalent in the population. It is multifactorial and originates from the formation of the bacterial plaque and its non-elimination by physical and chemical hygiene methods.
The use of chemical agents to aid in the control of Bacterial Plaque has been the subject of study in many investigations. Today there is a variety of elements that can be use to carry out proper oral hygiene, such as toothbrushes, toothpastes, dental floss and mouthwash, all of them in a wide variety.
Often the lack of accessibility to these means problems in the hygiene in the population, either due to the lack of knowledge or the cost of obtaining them.
Therefore, the objective of analyzing the antibacterial effect of the infusion of green tea (camellia sinensis) used as a mouthwash on saliva and bacterial plaque was presented. The proposed research topic is relevant, since if green tea (camellia sinensis) is found to produce positive effects on bacterial plaque, it would serve to prevent its formation, thus helping to improve dental hygiene with an easy acquisition product and lower cost compared to other mouthwashes offered in the market, constituting a natural mouthwash.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Prevention
- 盲法
- Single (Participant)
盲法说明
It was single blind, since the patient did not wat treatment was receiving. It was used a placebo.
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Participants that signed the informed consent
排除标准
- •allergic to green tea (Camellia sinensis)
- •ongoing medical or dental treatment that could interfere with the results.
研究组 & 干预措施
GREEN TEA MOUTH WASH
an infusion at 13%, with 13 g of green tea (commercially divided) in 100 ml of saline solution, at a temperature of approximately 90 ° C.
干预措施: Green Tea mouthwash (Other)
Placebo
distilled water, in sterile glass containers.
干预措施: Placebo Mouthwash (Other)
结局指标
主要结局
Antimicrobial effect
时间窗: up to 24 hours, thorough study completion
The measure was done with the difference in count of Colony forming units before and after intervention
Bacterial plaque Stage 1 (Before intervention)
时间窗: thorough study completion, an average of 24 hours
The measure was done with the count of Colony forming units
Bacterial plaque Stage 2 (After intervention)
时间窗: 1 minute after mouthwash use
The measure was done with the count of Colony forming units
次要结局
未报告次要终点
研究者
Julieta María Méndez Romero
Head of Writting and Publication Department, Regional Health Research Institute
Universidad Nacional de Caaguazu