Zinc and the Synthesis of Zinc Binding Proteins (Protocol B)
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Serum zinc concentration
研究概览
简要总结
The specific aim of this protocol is to examine differential expression of specific mRNAs and proteins that occur when the dietary intake of zinc is acutely reduced below the dietary requirement for a period of ten days.
详细描述
Methods to assess the dietary zinc status of individual humans or populations are lacking. The study is a three period observational study of biomarkers of zinc status before, during and after zinc depletion. The entire dietary study will take 24 days composed of three parts:
- Dietary Acclimation (Phase 1): 7 days
- Dietary Zinc Restriction (Phase 2): 10 days
- Dietary Zinc Repletion (Phase 3): 7 days
The dietary treatments and all blood draws will be carried out in the General Clinical Research Center (GCRC) at University of Florida. Previous research from has shown that both metallothionein gene expression and specific zinc transporter genes in blood leukocyte subsets are directly proportional to dietary zinc intake. By using cDNA array analysis and quantitative PCR technology with blood RNA, those genes most sensitive to zinc nutrition will be identified for the purpose of developing zinc status assessment methods on how zinc functions in human biology.
Additionally, buccal mucosal cells will be collected in the GCRC by using swabs, i.e., in a noninvasive manner, to further characterize the expression pattern of the genes under study. This method has the potential for nutrition studies in the field (international nutrition, etc).
研究设计
- 研究类型
- Observational
- 时间视角
- Prospective
入排标准
- 年龄范围
- 21 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Healthy male
- •21-35 years
- •Body weight above 110 lb
排除标准
- •Use of dietary supplements
- •Alcoholic
- •Under medication
- •History of chronic disease
- •Use of denture cream
- •Vegetarian
- •Food allergy
结局指标
主要结局
Serum zinc concentration
时间窗: 24 days
次要结局
未报告次要终点