Skeletal Muscle Lipid Metabolism and Insulin Sensitivity: Can Dietary Fatty Acid Composition Modulate Muscle Lipid Storage?
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Acute effect of meals with various fatty acid composition on postprandial insulin sensitivity
研究概览
简要总结
Rationale: The investigators hypothesize that a change in dietary fat quality (an increase in unsaturated fatty acids) may modulate fuel partitioning within the type 2 diabetic muscle towards less accumulation of lipid metabolites and an improved insulin sensitivity.
Objective:
- The contribution of dietary vs endogenous fat sources to lipid overflow in the circulation and to skeletal muscle lipid uptake and storage in obese insulin resistant subjects vs obese insulin sensitive controls.
- The acute effect of meals with various fatty acid composition ((high saturated (SFA) vs polyunsaturated (PUFA) vs monounsaturated (MUFA)) on skeletal muscle lipid uptake and storage, fatty acid mediated gene expression and postprandial insulin sensitivity in obese insulin resistant subjects.
Study design: single-blind randomized cross-over trial
Study population: obese males (35-70 years) with or without insulin resistance
Intervention:
- 1 test meal
- 3 test meals with a different fatty acid composition
Main study parameters/endpoints:
More insight whether dietary fat quality may modulate circulating lipids and skeletal muscle fatty acids handling, and may affect oxidative capacity and lipid storage in parallel to an increased insulin sensitivity.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Prevention
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 35 Years 至 70 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Obese males aged between 35 and 70 years with insulin resistance.
排除标准
- •History of type 2 diabetes.
- •Liver or kidney failure.
- •Diseases in which the live expectation is shorter then 5 years.
- •The use of experimental medication.
- •Diseases/medication use that may have an influence on the glucose tolerance.
- •Not to be able to understand the study information.
- •Users of fatty acid supplements including fish oils etc.
- •Users of high doses of antioxidant vitamins.
- •Athletes.
- •People on a diet of people that are planning to lose weight.
结局指标
主要结局
Acute effect of meals with various fatty acid composition on postprandial insulin sensitivity
时间窗: the individual participants will be measured within 8-10 weeks.
The acute effect of meals with various fatty acid composition ((high saturated (SFA) vs polyunsaturated (PUFA) vs monounsaturated (MUFA)) on postprandial insulin sensitivity will be measured during the stable isotope test.
Acute effect of meals with various fatty acid composition on forearm muscle fatty acid handling
时间窗: the individual participants will be measured within 8-10 weeks.
Using the stable isotope technique, we can differentiate between the metabolic fate of dietary versus endogenous fatty acids
次要结局
- Acute effect of meals with various fatty acid composition on intramuscular fatty acid handling(the individual participants will be measured within 8-10 weeks.)
- Acute effect of meals with various fatty acid composition on transcriptional regulation of the fatty acid metabolism in skeletal muscle biopsies(the individual participants will be measured within 8-10 weeks.)