Insulin and Muscle Fat Metabolism
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 入组人数
- 9
- 试验地点
- 2
- 主要终点
- Changes in skeletal muscle lipid content during hyperinsulinemia compared with basal resting conditions
研究概览
简要总结
Our objective in this study is to identify the extent to which insulin drives the accumulation of lipids in skeletal muscle of humans. We will test the hypothesis that 4-hours of mild hyperinsulinemia will result in significant muscle lipid accumulation and that such effects will be similar in lean and overweight/obese humans.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 30 Years 至 55 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age 30-55 years
- •Body mass index (BMI) 18-45 kg/m2
- •Sedentary (<1 hour of planned exercise per week)
排除标准
- •Regular exercise (>1 hour of planned exercise per week)
- •Smoking, tobacco or nicotine use within the last 1-year
- •Fasting glucose >126mg/dL
- •Hypertension (systolic pressure >140 mmHg or diastolic pressure >90 mmHg)
- •Chronic metabolic or cardiovascular health conditions
- •Pregnant, nursing, irregular menses or post-menopausal
- •Lidocaine allergy
- •Certain medications
- •Diminished capacity for consent
结局指标
主要结局
Changes in skeletal muscle lipid content during hyperinsulinemia compared with basal resting conditions
时间窗: Muscle samples will be collected in basal and hyperinsulinemic conditions separated by ~4.5 hours during the metabolic study visit
Liquid chromatography tandem mass spectrometry (targeted lipidomics) will be used to assess species-level changes in skeletal muscle lipid content in biopsy samples collected before and after the insulin infusion to induce mild hyperinsulinemia.
次要结局
- Changes in skeletal muscle mitochondrial oxidative capacity during hyperinsulinemia compared with basal resting conditions(Muscle samples will be collected in basal and hyperinsulinemic conditions separated by ~4.5 hours during the metabolic study visit)
研究者
Sean Newsom
Associate Professor
Oregon State University