Insulin and Muscle Fat Metabolism
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Overweight and Obesity
- Sponsor
- Oregon State University
- Enrollment
- 9
- Locations
- 1
- Primary Endpoint
- Changes in skeletal muscle lipid content during hyperinsulinemia compared with basal resting conditions
- Status
- Terminated
- Last Updated
- 3 years ago
Overview
Brief Summary
Our objective in this study is to identify the extent to which insulin drives the accumulation of lipids in skeletal muscle of humans. We will test the hypothesis that 4-hours of mild hyperinsulinemia will result in significant muscle lipid accumulation and that such effects will be similar in lean and overweight/obese humans.
Investigators
Sean Newsom
Associate Professor
Oregon State University
Eligibility Criteria
Inclusion Criteria
- •Age 30-55 years
- •Body mass index (BMI) 18-45 kg/m2
- •Sedentary (\<1 hour of planned exercise per week)
Exclusion Criteria
- •Regular exercise (\>1 hour of planned exercise per week)
- •Smoking, tobacco or nicotine use within the last 1-year
- •Fasting glucose \>126mg/dL
- •Hypertension (systolic pressure \>140 mmHg or diastolic pressure \>90 mmHg)
- •Chronic metabolic or cardiovascular health conditions
- •Pregnant, nursing, irregular menses or post-menopausal
- •Lidocaine allergy
- •Certain medications
- •Diminished capacity for consent
Outcomes
Primary Outcomes
Changes in skeletal muscle lipid content during hyperinsulinemia compared with basal resting conditions
Time Frame: Muscle samples will be collected in basal and hyperinsulinemic conditions separated by ~4.5 hours during the metabolic study visit
Liquid chromatography tandem mass spectrometry (targeted lipidomics) will be used to assess species-level changes in skeletal muscle lipid content in biopsy samples collected before and after the insulin infusion to induce mild hyperinsulinemia.
Secondary Outcomes
- Changes in skeletal muscle mitochondrial oxidative capacity during hyperinsulinemia compared with basal resting conditions(Muscle samples will be collected in basal and hyperinsulinemic conditions separated by ~4.5 hours during the metabolic study visit)