Insulin and Muscle Fat Metabolism

Not Applicable

Terminated

• Conditions: Overweight and Obesity; Sedentary Lifestyle; Insulin Resistance; Metabolic Disease; Mitochondrial Metabolism
• Interventions: Other: Hyperinsulinemic-euglycemic Clamp

• Registration Number: NCT04759872
• Lead Sponsor: Oregon State University

Brief Summary

Our objective in this study is to identify the extent to which insulin drives the accumulation of lipids in skeletal muscle of humans. We will test the hypothesis that 4-hours of mild hyperinsulinemia will result in significant muscle lipid accumulation and that such effects will be similar in lean and overweight/obese humans.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2021-02-09
• Study First Submitted QC: 2021-02-15
• Study First Posted: 2021-02-18
• Study Start: 2021-02-22
• Status Verified: 2022-06
• Primary Completion: 2022-06-07
• Study Completion: 2022-06-07
• Last Update Submitted: 2022-06-07
• Last Update Posted: 2022-06-10

Recruitment & Eligibility

• Status: TERMINATED
• Sex: All
• Target Recruitment: 9

Inclusion Criteria

• Age 30-55 years
• Body mass index (BMI) 18-45 kg/m2
• Sedentary (<1 hour of planned exercise per week)

Exclusion Criteria

• Regular exercise (>1 hour of planned exercise per week)
• Smoking, tobacco or nicotine use within the last 1-year
• Fasting glucose >126mg/dL
• Hypertension (systolic pressure >140 mmHg or diastolic pressure >90 mmHg)
• Chronic metabolic or cardiovascular health conditions
• Pregnant, nursing, irregular menses or post-menopausal
• Lidocaine allergy
• Certain medications
• Diminished capacity for consent

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Metabolic Study Visit	Hyperinsulinemic-euglycemic Clamp	Participants will complete a study visit for metabolic phenotyping and determination of the impact of hyperinsulinemia on outcomes of interest.

Primary Outcome Measures

Name	Time	Method
Changes in skeletal muscle lipid content during hyperinsulinemia compared with basal resting conditions	Muscle samples will be collected in basal and hyperinsulinemic conditions separated by ~4.5 hours during the metabolic study visit	Liquid chromatography tandem mass spectrometry (targeted lipidomics) will be used to assess species-level changes in skeletal muscle lipid content in biopsy samples collected before and after the insulin infusion to induce mild hyperinsulinemia.

Secondary Outcome Measures

Name	Time	Method
Changes in skeletal muscle mitochondrial oxidative capacity during hyperinsulinemia compared with basal resting conditions	Muscle samples will be collected in basal and hyperinsulinemic conditions separated by ~4.5 hours during the metabolic study visit	High-resolution respirometry will be used to assess changes in skeletal muscle mitochondrial oxidative capacity in biopsy samples collected before and after the insulin infusion to induce mild hyperinsulinemia.

Trial Locations

Locations (1)

Oregon State University; 🇺🇸 Corvallis, Oregon, United States