Skeletal Muscle Protein Metabolism and Insulin Sensitivity in Overweight Individuals: Effects of Meals With Various Fatty Acid Compositions

Not Applicable

Completed

• Conditions: Insulin Resistance
• Interventions: Dietary Supplement: Placebo; Dietary Supplement: Saturated fat; Dietary Supplement: Fish Oil

• Registration Number: NCT03146286
• Lead Sponsor: University of Nottingham

Brief Summary

The aim of the study will investigate whether impairment in the action of insulin to promote the use of glucose in skeletal muscle (insulin resistance) as a result of oral ingestion of a liquid meal rich in saturated fat is linked to reduced ability of muscles to synthesise new protein in response to dietary protein intake, which ultimately may compromise maintenance of muscle size and quality of life and whether partially replacing saturated fat in the liquid meal with omega 3 polyunsaturated FA (n3PUFA) will ameliorate these negative effects.

Detailed Description

Not available

Study Timeline

• Study Start: 2015-08-01
• Primary Completion: 2015-12-03
• Study Completion: 2015-12-03
• Study First Submitted: 2017-05-05
• Study First Submitted QC: 2017-05-08
• Study First Posted: 2017-05-09
• Status Verified: 2019-05
• Last Update Submitted: 2019-05-17
• Last Update Posted: 2019-05-21

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 8

Inclusion Criteria

• Male, healthy, overweight (BMI>27 kg/m2 and fat mass >30%) non-diabetic individuals.

Exclusion Criteria

• Diabetes, smoking, cardiovascular disease

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Control	Placebo	Chocolate flavoured drink spiked with 15mg/kg \[2H31\]palmitate (D31palmitate) and singly-labelled water (H218O). 4 hours post test meal a bolus of milk protein (30 g), which has been intrinsically labelled with \[13C\]phenylalanine, along with 75 g of glucose in 250 ml of water will be given.
Saturated Fat	Saturated fat	Chocolate flavoured drink spiked with 15mg/kg \[2H31\]palmitate (D31palmitate) and singly-labelled water (H218O) containing 0.7 g/kg bodyweight palm stearin. 4 hours post test meal a bolus of milk protein (30 g), which has been intrinsically labelled with \[13C\]phenylalanine, along with 75 g of glucose in 250 ml of water will be given.
Fish Oil	Fish Oil	Chocolate flavoured drink spiked with 15mg/kg \[2H31\]palmitate (D31palmitate) and singly-labelled water (H218O) containing 0.45 g/kg bodyweight palm stearin and 0.25 g/kg bodyweight fish oil (n3PUFA). 4 hours post test meal a bolus of milk protein (30 g), which has been intrinsically labelled with \[13C\]phenylalanine, along with 75 g of glucose in 250 ml of water will be given.

Primary Outcome Measures

Name	Time	Method
Change in muscle protein fractional synthetic rate	3 hours post protein drink	Measurement of FSR at baseline and post intervention

Secondary Outcome Measures

Name	Time	Method
whole body postprandial insulin sensitivity	Baseline and every 15mins for 7 hours	Glucose and insulin area under the curves (AUC) in response to the milk protein and glucose ingestion will be measured
Whole body and skeletal muscle fat oxidation	Baseline and every 15mins for 7 hours	D31palmitate plasma enrichment and incorporation into skeletal muscle lipids