Myofibroblastic Transformation Secondary to Epithelial-stromal Interactions in the Keratoconus (MYKE)
试验速览
- 阶段
- 不适用
- 状态
- 撤回
- 主要终点
- Comparison of alpha-SMA's (Smooth Muscle Actin) messenger RNA expression evaluated by quantitative PCR (RT-qPCR) in corneal stroma in keratoconus patients compared to non-keratoconus controls
研究概览
简要总结
Keratoconus is characterized by a thinning of the cornea, which causes a decrease in visual acuity due to astigmatism.
Publications suggest that keratoconus is linked to chronic inflammation (increase in pro-inflammatory cytokines and metalloproteinases (MMP). Direct epithelial-stromal interactions (D-ESI) have a role in the induction of metalloproteinases (MMP) and the differentiation of fibroblasts into myofibroblasts via an EMMPRIN membrane glycoprotein (extracellular matrix membran MMP inducer - CD 147). On a healthy cornea, EMMPRIN's effects are prevented by a lack of contact between epithelial and stromal cells through a basement membrane, which is altered in the keratoconus The hypothesis is that stromal thinning of the keratoconus could be related to increased expression of EMMPRIN by epithelial and stromal cells (resulting in increased MMP synthesis), with a preponderance at the most deformed areas.
The main objective is to demonstrate a transformation of fibroblasts to myofibroblasts in the corneal stroma of keratoconus patients.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •For the cases :
- •Suffering from keratoconus and requiring a first optical corneal transplant
- •For the controls:
- •Orbital exenteration operation due to an orbital tumor
- •Absence of any anomaly of the ocular surface observed during the slit lamp examination at the last preoperative consultation
排除标准
- •For the cases:
- •Keratoconus patient requiring a tectonic corneal transplant
- •Known pregnancy, or breastfeeding
- •For the controls:
- •History of orbital radiotherapy
- •History of corneal surgery
- •History of corneal surface tumour
- •Eye surface abnormality noted in the preoperative period
- •Known keratoconus
- •Known pregnancy, or breastfeeding
- •Secondary exclusion if a keratoconus is diagnosed during the immunohistochemical analysis by visualization of Bowman membrane interruption
结局指标
主要结局
Comparison of alpha-SMA's (Smooth Muscle Actin) messenger RNA expression evaluated by quantitative PCR (RT-qPCR) in corneal stroma in keratoconus patients compared to non-keratoconus controls
时间窗: 12 hours
次要结局
未报告次要终点