The Combined Effects of Whey Protein and Collagen Supplementation on Bone Mineral Density and Muscle Mass in Resistance-Trained Men: A Randomized Controlled Trial
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Change in Muscle Mass (kg, lean body mass by BIA)
研究概览
简要总结
This study tested whether taking whey protein together with collagen peptides would provide greater benefits for muscle and bone health compared to whey protein alone, collagen alone, or a placebo. Forty healthy, resistance-trained men aged 18-35 years were randomly assigned to one of four groups: (1) whey protein plus collagen (30 g + 10 g/day), (2) whey protein only (30 g/day), (3) collagen only (10 g/day), or (4) placebo (maltodextrin). All participants followed a supervised resistance training program (3 times per week) for 8 weeks.
The primary outcome was muscle mass, measured with bioelectrical impedance analysis (BIA). Secondary outcomes included bone mineral density (DXA scans), maximal strength (1RM squat and bench press), and blood markers of bone turnover (P1NP and CTX-I).
Results showed that the whey + collagen group achieved the largest improvements in muscle growth, lumbar spine bone mineral density, strength, and favorable changes in bone turnover markers compared to all other groups. No serious side effects were reported, and supplement adherence was very high.
These findings suggest that combining whey protein and collagen may be a practical strategy to support muscle and bone adaptation in resistance-trained men.
详细描述
This was a double-blind, randomized, placebo-controlled trial evaluating the combined effects of whey protein and collagen supplementation on muscle mass, bone mineral density (BMD), muscular strength, and bone turnover markers in resistance-trained men.
Forty healthy male participants (18-35 years, with ≥1 year of resistance training experience) were recruited and randomized into four parallel groups (n=10 per group):
Whey Protein + Collagen (30 g WP + 10 g collagen/day)
Whey Protein only (30 g/day)
Collagen only (10 g/day)
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
盲法说明
Double-blind design extended to all relevant parties. Supplements were provided in identical, opaque sachets to ensure concealment. Randomization sequence was managed by an independent statistician not involved in data collection or analysis. Participants, trainers, investigators, and outcome assessors remained blinded to group assignments until completion of analyses.
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Male participants aged 18-35 years
- •At least 1 year of consistent resistance training experience (≥3 sessions per week, targeting major muscle groups)
- •Healthy, with no history of musculoskeletal, metabolic, or cardiovascular disorders
- •No current or recent (<6 months) use of anabolic steroids, growth hormones, or performance-enhancing substances
- •Non-smoker and no habitual use of anti-inflammatory medications
- •Able and willing to comply with supplementation and supervised training program
- •Provided written informed consent
排除标准
- •Participation in similar supplementation studies in the past 6 months
- •Recent fractures or surgeries affecting musculoskeletal health (<6 months)
- •Known allergy or intolerance to dairy proteins or collagen supplements
- •Inability to attend scheduled supervised resistance training sessions
- •Non-adherence to dietary control requirements (e.g., use of additional protein/creatine supplements during study)
- •Any condition deemed by investigators to interfere with study compliance or safety
研究组 & 干预措施
Whey Protein + Collagen (WP+C)
Participants received 30 g/day whey protein isolate plus 10 g/day type I & III collagen peptides, split into two daily doses (morning and post-exercise or at same time on rest days). Supplements were packaged in identical sachets.
干预措施: Whey Protein + Collagen (Dietary Supplement)
Collagen Only (C)
Participants received 10 g/day type I & III collagen peptides, split into two daily doses. Identical packaging ensured blinding.
干预措施: Collagen Peptides (Dietary Supplement)
Placebo (P)
Participants received 10 g/day maltodextrin (isocaloric with protein groups), provided in identical sachets to ensure blinding.
干预措施: Maltodextrin (Placebo) (Other)
Whey Protein Only (WP)
Participants received 30 g/day whey protein isolate, split into two daily doses. Packaged identically to other interventions to maintain blinding.
干预措施: Whey Protein (Dietary Supplement)
结局指标
主要结局
Change in Muscle Mass (kg, lean body mass by BIA)
时间窗: Baseline (week 0) to 8 weeks post-intervention
Muscle mass was assessed using bioelectrical impedance analysis (InBody 770). Measurements were conducted under standardized conditions (≥8 h fasted, no exercise or caffeine for ≥12 h, urine specific gravity \<1.020). Values reported as lean body mass (kg). Primary analysis compared changes between Whey + Collagen, Whey only, Collagen only, and Placebo groups after 8 weeks of supplementation combined with resistance training.
次要结局
- Change in Lumbar Spine Bone Mineral Density (BMD)(Baseline to 8 weeks)
- Change in Femoral Neck Bone Mineral Density (BMD)(Baseline to 8 weeks)
- Change in Squat Strength (1RM)(Baseline to 8 weeks)
- Change in Bench Press Strength (1RM)(Baseline to 8 weeks)
- Change in Procollagen Type I N-Terminal Propeptide (P1NP)(Baseline to 8 weeks)