Inflammation, Intracellular Invasion and Colonization of the Nasal Mucosa by Staphylococcus Aureus
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 157
- 试验地点
- 2
- 主要终点
- level of ICAM-1 expression in nasal cells
研究概览
简要总结
An inflammatory state of the nasal cells (very close to keratinocytes) could favour the internalization of Staphylococcus aureus and thus constitute a persistent reservoir for the carriage of this bacterium.
Staphylococcus aureus is a commensal bacterium of the skin and mucous membranes that colonizes approximately 2 billion people worldwide Staphylococcus aureus is also a leading cause of community and healthcare-associated infection. Staphylococcus aureus has demonstrated its ability to invade many non-professional phagocytic cell lines such as keratinocytes, osteoblasts, fibroblasts, epithelial cells and endothelial cells. During pro-inflammatory stimulation, internalization of Staphylococcus aureus into keratinocytes is mainly mediated by ICAM-1. These results suggest that, in humans, an inflammatory state of the nasal cells (very close to keratinocytes) could promote the internalization of Staphylococcus aureus and thus constitute a persistent reservoir for the carriage of this bacterium.
详细描述
The main objective is to compare the expression level of ICAM-1 in nasal cells with and without the presence of Staphylococcus aureus.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Subject affiliated to a social security scheme or entitled person
- •Subject who received informed information about the study et signed consent
- •Adult subject (≥18ans)
- •Healthy Volunteer
排除标准
- •Subject undergoing antibiotic treatment or having received antibiotic treatment (oral, IV or local) in the month prior to inclusion
- •Subject with a major haemostasis disorder or anticoagulant treatment contraindicated for surgery
- •Subject with a pathology increasing the nasal carriage of S. aureus (chronic furunculosis, diabetes, HIV infection, chronic rhinosinusitis)
- •Subject under guardianship or trusteeship
- •Pregnant woman
结局指标
主要结局
level of ICAM-1 expression in nasal cells
时间窗: Months: 0
Compare the level of ICAM-1 expression in nasal cells with and without the presence of intracellular Staphylococcus aureus
次要结局
- concentration of ICAM-1 in S. Aureus(Months: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- Measurement of the expression of the genes (mRNA dosage) involved in IL-1 beta secretion(Months: 0)
- Measurement of the expression of the genes (mRNA dosage) involved in activation of autophagy(Months: 0)
- cytokine concentration(Months: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)