The Impact of Protein Supplementation to Maximize Skeletal Muscle Endurance Training Adaptations in Healthy Young Men
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 44
- 试验地点
- 2
- 主要终点
- Maximal whole-body oxidative capacity
研究概览
简要总结
Participants will perform three endurance training sessions weekly for 12 weeks. All participants will be randomly assigned to the protein supplementation group or the placebo (iso-caloric carbohydrate) group. During the 12 weeks endurance training program participants allocated to the protein group (30 g protein per beverage) will receive a protein drink after exercise and prior to sleep at night on training days. On non-training days participants will only receive a protein drink before sleep. Participants allocated to the carbohydrate group (30 g carbohydrates) will receive a carbohydrate drink instead of a protein drink.
详细描述
In the proposed study, the effects of protein supplementation after training and before sleep on VO2max and performance will be assessed during 12 weeks endurance exercise training in untrained healthy young men. The ready to drink carbohydrate beverage consists of 30g carbohydrate or 30g protein. Previous studies show that the supplementation of 30g protein is in the optimal range to stimulate the muscle protein synthetic response following resistance type exercise. The protein supplementation group in the present study will be compared with an iso-caloric carbohydrate group. In addition, to the pre- and post-intervention biopsies 1 additional muscle biopsies will be taken (in week 6) during the 12 week intervention period. This will create a unique opportunity to establish the timeline of the skeletal muscle adaptive response at functional and mechanistic level following prolonged endurance exercise training, including potential differences between both groups.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Investigator)
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Age between 18 and 30 years of age
- •BMI between 18.5 - 25 kg/m2
- •Recreationally active, performing sports on a non-competitive basis
- •Maximal wattage of > 5 watt per kg bodyweight (screening measurement)
- •Willing to give muscle biopsies
- •Willing to give blood samples
- •Having a general-practitioner
- •Consumption of alcohol beverages is less than 21 per week
- •Able to perform three exercise sessions weekly for 10 weeks
排除标准
- •Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, liver or renal disease, diabetes mellitus type 1 or 2)
- •Having a lactose and/or gluten intolerance
- •Use of systemic medication (with the exception of antihistaminic medication and the use of occasional painkillers)
- •(Chronic) injuries of the locomotor system (e.g. musculoskeletal/orthopedic disorders) that can interfere with the intervention
- •Participants who are enrolled in an interventional biomedical research project or have received an investigational new drug or product with the last 30 days prior to screening.
- •Smokers and use of illicit drugs
- •Blood donor during the study and in the three months before start of the study
- •Employed, or intern, or working on a thesis at the department of Human Nutrition at Wageningen University & Research
- •Participating in another scientific study (except EetMeetWeet)
研究组 & 干预措施
Protein
Three endurance training sessions weekly with protein supplementation post-exercise and before sleep.
干预措施: Protein (Dietary Supplement)
Carbohydrate
Three endurance training sessions weekly with carbohydrate supplementation post-exercise and before sleep.
干预措施: Protein (Dietary Supplement)
结局指标
主要结局
Maximal whole-body oxidative capacity
时间窗: 12 weeks
Maximal oxygen consumption in ml per kg of bodyweight
次要结局
- Lean mass(12 weeks)
- Endurance Performance(12 weeks)
- Maximal activity of citrate synthase(12 weeks)
- Maximal activity of Cytochrome c oxidase(12 weeks)
- Erythrocytes(12 weeks)
- Hemoglobin(12 weeks)
- Hematocrit(12 weeks)
- Fat mass(12 weeks)