Advanced Sperm Selection Techniques and Their Contribution to Blastocyst Euploidy Rates
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 515
- 试验地点
- 2
- 主要终点
- Aneuploidy rate
研究概览
简要总结
Comparing different advanced sperm selection techniques like Physiological ICSI (PICSI) and magnetic activated cell sorting (MACS) in terms of the PGT-A outcomes of each arm blastocysts
详细描述
Advanced sperm selection methods like PICSI and MACS have been developed for selecting a healthy mature non apoptotic sperm with lower Sperm DNA fragmentation. Previous studies compared the pre-implantation embryo development parameters of those techniques, but none looked at comparing the PGT-A results or euploidy rates of the blastocysts derived from each sperm selection.
PGT-A cases will be randomized on the day of ICSI to the 2 assigned sperm selection techniques.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Case must have PGT-A for all of her blastocysts
- •Males diagnosed with abnormal sperm DNA fragmentation index (> 20%).
- •Normo responder (> 5 mature oocytes)
- •Male will have to refrain from ejaculation no less than 1 day but no greater than 3 days prior semen specimen production on day of ICSI
排除标准
- •Leukocytospermia
- •Presence of varicocele.
- •Known genetic abnormality
- •Use of sperm or oocyte donors
- •Use of gestational carrier
- •Presence of any of the endometrial factors that affect embryo implantation such as hydrosalpings, adenomyosis or previous uterine infection
- •Any contradictions to undergoing in vitro fertilization or gonadotropin stimulation
结局指标
主要结局
Aneuploidy rate
时间窗: 15 days post ICSI
Defined as the proportion of aneuploid blastocysts
Euploidy rate
时间窗: 15 days post ICSI
Defined as the proportion of euploid blastocysts
Low mosaic rate
时间窗: 15 days post ICSI
Defined as the proportion of low mosaic blastocysts
High mosaic rate
时间窗: 15 days post ICSI
Defined as the proportion of high mosaic blastocysts
次要结局
- Cleavage rate(3 days)
- Fertilization rate(1 day)
- Blastocyst development rate(5-6 days)
- Blastocyst quality rate(5-6 days)