Methodological Evaluation of Gingival Crevicular Fluid Volume and Elastase Levels: Sequential Sampling, Length of Sampling Time And Two Different Sampling Methods
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 主要终点
- The length of sampling time of GCF Elastase Levels
研究概览
简要总结
The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme activity by using intracrevicular and orifice methods.
详细描述
Twenty adults (mean age of 41.8 years. ranged 31-60 years. 18 males. 2 females) with chronic periodontitis were enrolled and all completed the 3-day study. Three-hundred-sixty samples of GCF were harvested from the interproximal sites of each of the two maxillary non-molar teeth according to the orifice method. Each site was sampled three times with 1-min intervals between repeat samples. By using intracrevicular method, the other 360 samples of GCF were collected on the same sites following 10-minutes interval. In first day, 2nd day and 3rd day the length of sampling time in seconds and order were 5. 10. 30; 10. 30. 5 and 30. 5. 10. respectively. GCF elastase activity was determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide and was expressed as concentration (microU/microl) and total enzyme activity (microU).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- Double (Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 31 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •no history of systemic disease;
- •no history of antibiotics and/or anti-inflammatory drugs within the past 6 months;
- •no received any oral hygiene prophylaxis and scaling and root planing within the past 6 months;
- •at least 2 interproximal sites exhibiting higher than 5 mm probing depth, radiographic bone loss and gingival index of 2 (with preference of two maxillary non-molar teeth)
排除标准
- •with metabolic diseases
- •pregnancy, lactation etc.
研究组 & 干预措施
the first day
Gingival crevicular fluid collection with filter paper the length of sampling time in seconds: 5., 10., 30.
干预措施: Gingival crevicular fluid collection with filter paper (Other)
the 2nd day
Gingival crevicular fluid collection with filter paper the length of sampling time in seconds: 10., 30., 5.
干预措施: Gingival crevicular fluid collection with filter paper (Other)
the 3rd day
Gingival crevicular fluid collection with filter paper the length of sampling time in second: 30., 5., 10.
干预措施: Gingival crevicular fluid collection with filter paper (Other)
结局指标
主要结局
The length of sampling time of GCF Elastase Levels
时间窗: 3 days
次要结局
- The length of sequential sampling of GCF Volume(3 days)
- The length of sampling time of GCF Volume(3 days)
- The length of sequential sampling of GCF Elastase Levels(3 days)
研究者
Nilgün Özlem Alptekin
Prof., DDS, PhD
Baskent University