Expression of miR-204 and Consequences on Capillarization in Limb Muscles of Patients With Chronic Obstructive Pulmonary Disease
试验速览
- 阶段
- 不适用
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- miR-204 in limb muscles in patients with COPD
研究概览
简要总结
Limb muscle dysfunction, characterized by loss of capillaries, is amongst the most troublesome systemic consequences of chronic obstructive pulmonary disease (COPD) leading to poor functional status and premature mortality.
One prevailing hypothesis stipulates that modification in the expression of miR-204 leads to change the regulation of angiogenesis in vastus lateralis of patients with COPD when compared to controls.
详细描述
The investigators objective is to investigate the impact of COPD on key signaling pathways involved in the muscle angiogenesis. Mild to severe COPD patients and healthy controls will be recruited. All of the investigators patients will undergo a thorough baseline assessment in pulmonary capacity and body composition. Blood sampling and biopsy of the quadriceps will be done. Key proteins and microRNAs of signaling intramuscular pathways involved in angiogenesis will be measured in the quadriceps.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 50 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •COPD (FEV1/FVC < 0.70)
- •Healthy subjects: no evidence of airways obstruction (FEV1/FVC > 0.70 and FEV1 > 0.80 predicted)
- •Smoking history > 10 pack-years
- •Ex-smoker >6 months
排除标准
- •Chronic hypoxemia and/or hypercapnia (PaO2 < 60 mmHg or SpO2 < 88% at rest and/or PaCO2 > 45 mmHg)
- •Recent exacerbation or oral corticosteroids (< 2 months)
- •Recent cancer (< 3 years)
- •Myopathy, neuromuscular, neurological or articular diseases
- •Kidney disease
- •Other respiratory diseases
- •Unstable cardiac disease
- •Physical activity score > 9 in the Voorips questionnaire
- •BMI > 30
结局指标
主要结局
miR-204 in limb muscles in patients with COPD
时间窗: At baseline (COPD patients in a stable state of the disease)
i)To measure miR-204 level in the quadriceps of patients with COPD in comparison to controls. ii) To study possible involvement of miR-204 on pathways regulating angiogenesis and on muscle microcirculation in these individuals.
次要结局
- microRNAs in limb muscles of patients with COPD(At baseline (COPD patients in a stable state of the disease))