Effect of Dietary Macronutrient Composition on Liver Substrate Metabolism
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 2
- 主要终点
- de novo lipogenesis
研究概览
简要总结
The purpose of this study is to understand why Hispanics who are overweight have a higher incidence of fatty liver disease.
详细描述
Obesity is a major factor driving the increased prevalence of hepatic steatosis in the US. However, little is known regarding the relationship between dietary intake and hepatic fat deposition or about the factors that promote loss of hepatic steatosis. Here, the investigators will determine how differences in dietary composition affect the development and regression of fatty liver. The investigators hypothesize that Hispanic subjects with metabolic syndrome will have higher liver fat synthesis rates compared to African American subjects.
Using detailed in vivo, serial measurements of fuel metabolism (GC/MS and NMR) fatty acid metabolism will be measured in the liver and periphery. This will be the first study in which these two methodologies are used together to assess both glucose and fatty acid metabolism in the same subjects. Subjects will be tested before and after a dietary weight-loss intervention producing 6% body weight loss over 5 months.
The specific aims are as follows:
AIM 1: Determine the contribution of peripheral and dietary fat to liver-TG in Hispanics and African Americans with metabolic syndrome.
Hypothesis: De novo lipogenesis will contribute to liver-TG in greater quantities compared to African Americans.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Factorial
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Elevated serum ALT or metabolic syndrome
- •African American or Hispanic
- •Nondiabetic
- •Men or women
- •Smokers and nonsmokers
- •Pre- and post-menopausal (+/- HRT)
- •Stable body weight
- •Age 20-65 years
- •BMI between 25-45 kg/m2
排除标准
- •Diabetes or Pregnancy
- •Ethanol intake: males > 140 g/week, females > 70 g/week
- •Chronic hepatitis B or chronic hepatitis C
- •Hemochromatosis or Wilson's Disease
- •Autoimmune hepatitis or primary biliary cirrhosis
研究组 & 干预措施
African American subjects
Subjects will self-identify as African American in origin.
干预措施: Low-fat diet (Other)
Hispanic subjects
Subjects will identify as Hispanic ethnicity.
干预措施: Low-fat diet (Other)
Hispanic subjects
Subjects will identify as Hispanic ethnicity.
干预措施: Low-carbohydrate diet (Other)
African American subjects
Subjects will self-identify as African American in origin.
干预措施: Low-carbohydrate diet (Other)
结局指标
主要结局
de novo lipogenesis
时间窗: Change from Baseline in fatty acid synthesis at 5 months
In vivo measurement is made of liver fatty acid synthesis using stable isotope administration and analysis of plasma samples by GS/MS
次要结局
- Adipose fatty acid flux(Change from Baseline in adipose fat flux at 5 months)
- Dietary fatty acid clearance to liver(Change from Baseline in dietary fat clearance at 5 months)
研究者
Elizabeth Parks
Associate Professor
University of Texas Southwestern Medical Center