Measure of Intestinal Lipogenesis in Healthy Volunteers and Obese Subjects Before and After Gastroplasty: Modulation by Glucose and Fructose
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 24
- 试验地点
- 1
- 主要终点
- Intestinal de novo lipogenesis (sub-study 1)
研究概览
简要总结
8 healthy volunteers, 8 pre-bypass obese subjects, 8 subjects between 12-18 months post-bypass, and 8 subjects matched to the post-bypass patients will be studied in two sub-studies Sub-study 1) after ingestion of two or three different of the following test-meals: A)cream and whey protein B)cream, whey protein and fructose + 13C6 fructose C)test meal with cream, whey protein, fructose and glucose + 13C6 fructose Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood Sub-study 2) after ingestion of two or three different of the following test-meals: A)cream labelled with 13C6 palmitic acid and whey protein B))cream labelled with 13C6 palmitic acid, whey protein, fructose and glucose
Their intestinal de novo lipogenesis will be estimated by measuring 13C-palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in blood in sub-study 1
Their exogenous lipid absorption kinetics will be assessed by measuring 13C palmitate on triglyceride-rich lipoprotein particles associated with apoB48 in sub-study 2
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 25 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •age 18-50 years
- •sex male or female
- •less than 2 kg body weight change over the past 3 months subjects
排除标准
- •antidiabetic and hypolipemic drugs
- •alcohol consumption >20g/day
- •severe eating disorders
- •severe psychological problems
- •vegetarian diet or other specific diet
- •consumption of illicit substances
- •pregnancy
- •weight gain or weight loss > 1 kg in the last 3 months
结局指标
主要结局
Intestinal de novo lipogenesis (sub-study 1)
时间窗: 0-8-hour post-prandial
Measurement of total TG and 13C palmitate in chylomicrons: The sf \>20 fraction of post-prandial blood samples will be prepared, and non-chylomicrons, apoB100-containing lipoprotein will be removes by immuno-affinity chromatography using a specific anti-apoB100 antibody
Postprandial exogenous, labelled palmitate concentration (sub-study 2)
时间窗: 0-8-hour post-prandial
Measurement of total TG and 13C palmitate in chylomicrons of sf \>20 fraction of post-prandial blood samples will be prepared,
次要结局
- post-prandial triglyceride profile (sub-studies 1 and 2(0-8 hour post-prandial)
研究者
Luc Tappy, MD
Professor
University of Lausanne