Evaluation of Adipose Tissue Pre and Post Bariatric Surgery
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 98
- 试验地点
- 1
- 主要终点
- Adipose tissue fibrosis using qRT-PCR, immunoblotting, histochemistry and immunohistochemistry
研究概览
简要总结
Published data suggest that inflammation and fibrosis of adipose tissue could be factors favoring the development of insulin resistance in obese individuals and that a decrease in the activity of the AMP-activity kinase protein (AMPK) could lead to these dysfunctions. However, very few data are available in humans.
There is also growing interest in persistent organic pollutants (POPs) as a cardiometabolic and type 2 diabetes (T2D) risk factor. There is some evidence to suggest that POPs directly contribute to lipid metabolism dysfunction and insulin resistance. Additionally, POPs are stocked in adipose tissue. The accumulation of POPs in adipose tissue therefore limits their bioavailability to other organs, thus reducing their systemic toxicity. It has been observed that a large amplitude weight loss leads to a significant increase in POPs in the blood.
The goal of this project is to identify adipose tissue factors/dysfunctions that contribute to insulin resistance and type 2 diabetes associated with obesity in humans and thus raise avenues for screening and treatment of these metabolic complications. More specifically, the objectives are:
- To study the relationship between AMPK, fibrosis and inflammation of adipose tissue and their role in the development of insulin resistance and T2D associated with obesity;
- To examine the relationship between POPs and the cardiometabolic profile.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Awaiting bariatric surgery
- •BMI ≥ 35 kg/m2 without cardiometabolic complications or ≥ 30 kg/m2 if presence of type 2 diabetes or hypertension
- •≥ 18 years old
- •Sedentary (less than 3 hours of regular physical activity per week)
排除标准
- •Type 1 diabetes
- •Acute event in the last 3 months (myocardial infarction, cerebrovascular hemorrhage or transient cerebral ischemia, unstable angina, peripheral vascular disease, revascularization or angioplasty, recent hospitalization for more than 4 days)
- •Infection in the last month (fever, antibiotics treatment)
- •Cancer in the last 3 years
- •Chronic inflammatory disease
- •Pharmacologic treatment (any medication affecting glucose metabolism (except those for diabetes), hypotensive medication unless stable dose for at least 1 month, lipid-lowering medication unless stable dose for at least 1 month)
- •Uncontrolled disease of pituitary or thyroid gland
- •Bleeding disorders
- •Alcohol or drug abuses
- •Claustrophobia
结局指标
主要结局
Adipose tissue fibrosis using qRT-PCR, immunoblotting, histochemistry and immunohistochemistry
时间窗: 6 months after bariatric surgery
次要结局
- Food quality using the Food Frequency Questionnaire(6 months after bariatric surgery)
- Persistant organic pollutants measured by high-resolution chromatography combined with high-resolution mass spectrometry(6 months after bariatric surgery)
- AMP-activated protein kinase(6 months after bariatric surgery)
- Calory intake(6 months after bariatric surgery)
- Inflammation in adipose tissue using qRT-PCR, immunoblotting and immunohistochemistry(6 months after bariatric surgery)
研究者
Rémi Rabasa-Lhoret
Full professor
Institut de Recherches Cliniques de Montreal