Effect of 2000-meter Rowing Test on Parameters of Intestinal Integrity in Elite Rowers During Competitive Phase
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 18
- 试验地点
- 1
- 主要终点
- LBP (lipopolysaccharide binding protein) to measure endotoxin
研究概览
简要总结
The study aimed to check the 2000m ergometer test on markers of gut permeability in the competitive phase of rowers. 18 members of the Polish rowing team took part in the study.
详细描述
The phenomenon of epithelial wall leakage has been the subject of investigations within athletic domains such as running and cycling. Nevertheless, there exists an insufficiency of understanding regarding gut permeability in alternative disciplines, such as rowing, particularly following the completion of a standard competitive distance of 2000 meters. Hence, the principal objective of our study was to assess gut permeability after completing a 2000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 2000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1-hour of restitution. Parameters, such as I-FABP, LPS, LBP, and zonulin, were determined using appropriate biochemical tests.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 23 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •minimum five years of training,
- •five training sessions per week minimum,
- •total training time minimum of 240 minutes,
- •membership in the Polish Rowing Team,
- •finishing the 2000-meter ergometer test.
排除标准
- •antibiotic therapy within the last three months,
- •probiotics and prebiotics within the last three months,
- •dietary regime,
- •gastrointestinal diseases.
结局指标
主要结局
LBP (lipopolysaccharide binding protein) to measure endotoxin
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LBP concentration measured in blood \[ug/ml\] using commercially available enzyme-linked immunosorbent assays
LPS (lipopolysaccharide) to measure endotoxin
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LPS concentration measured in blood \[EU/Ll\] using commercially available enzyme-linked immunosorbent assays
zonulin to measure tight junction leakage
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
zonulin concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
Lactic acid to measure fatigue after the race
时间窗: Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
Lactate concentration measured in capillary blood from earlobe \[mmol/L\] using a commercially available kit (Diaglobal, Berlin, Germany)
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
时间窗: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
I-FABP concentration measured in blood \[ng/ml\] using commercially available enzyme-linked immunosorbent assays
次要结局
- fiber intake(day before test)
- protein(day before test)
- Lean body mass(in the morning before the test)
- energy(day before test)
- carbohydrate(day before test)
- body mass(in the morning before the test)
- Body composition - fat(in the morning before the test)
- Body composition - water(in the morning before the test)