Comparative Microbial Epidemiology and Chlorhexidine Suscebtibily of Oropharyngeal and Intestinal Colonization of Healthy Adults Subjects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- proportion of patients with an oropharyngeal gram-negative colonization
研究概览
简要总结
In this prospective observational study we aim to determine the prevalence of oropharyngeal and rectal Gram-negative bacilli colonization in healthy subjects, and their susceptibility to chlorehexidine. We plan to recruit 100 healthy volunteers secondary endpoints are to determine the phylogentic characteristics of E. coli isolates; to compare the phylogentic characteristics of oropharyngeal and rectal of predominant GNB colonization.
详细描述
In this prospective observational study we aim to determine the prevalence of oropharyngeal and rectal Gram-negative bacilli colonization in 100 healthy subjects, and their susceptibility to chlorehexidine. Each participant will undego a oropharyngeal swab collection and a rectal swab at day 0. The Gram-negative bacilli colonization will be identified, E. coli isolates will be studied in terms of phylogeny; gram-negative isolates will be studied in terms of antimicrobial and chlorhexidine susceptibility. The primary objective is to determine the prevalence of oropharyngeal Gram-negative bacilli colonization. The secondary objectives are to determine the chlorhexidine and antimicrobial susceptibility of such colonization; to determine the phylogentic characteristics of oropharyngeal and rectal predominant E. coli isolates; to compare the phylogentic characteristics of oropharyngeal and rectal E. coli isolates.
研究设计
- 研究类型
- Observational
- 观察模型
- Ecologic Or Community
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •age > 18 yo
- •personal history of neck or face irradiation
- •no history of gastrointestinal disease
- •no symptoms of immunosuppression
- •no antibiotic therapy in the previous month
- •no hospitalization in the 3 months preceding inclusion
- •medical exmination prior to inclusion
排除标准
- •affiliated to the social security
- •The refusal of a patient to participate in a study
结局指标
主要结局
proportion of patients with an oropharyngeal gram-negative colonization
时间窗: Day 1
次要结局
- characterization of antimicrobial and chlorhexidine susceptibility of Gram negative bacteria from oropharyngeal colonization(Day 1)
- comparision of the predominant fecal flora with GNB oropharyngeal colonization(Day 1)
- phylogenetic and virulence factor content of E. coli oropharyngeal and rectal colonization(Day 1)
- incidence of the a GNB oropharyngeal colonization(Day 1)