The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization Outcome of Sibling Oocytes
试验速览
- 阶段
- 不适用
- 入组人数
- 600
- 试验地点
- 1
- 主要终点
- Fertilization Rate
研究概览
简要总结
The importance of glucose in culture media on fertilization and embryo development is still controversial. Although it has been reported that glucose is essential for fertilization process and biosynthesis of membrane lipid and nucleic acids, comparatively high levels of glucose in culture media may cause developmental arrest of cleavage stage embryos. The aim of this retrospective study is to examine whether exposure of sibling oocytes to different concentrations of glucose in culture media has any further effect on IVF outcome.
详细描述
This retrospective study will include data from either standard IVF or Intracytoplasmic Sperm Injection (ICSI) cycles, in which sibling oocytes were equally divided and incubated separately in one of the following pairs of culture media:
- Universal IVF medium vs. ISM1 medium ( Origio, Medi-Cult)
- P1 medium vs. ECM medium (Irvine).
Fertilization rate,as well as embryo cleavage rate and morphology will be evaluated.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 42 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Sibling oocytes from cycles where at least 4 oocytes were retrieved
排除标准
- •Less than 4 oocytes retrieved
- •Partner has undergone testicular sperm extraction (TESE)
结局指标
主要结局
Fertilization Rate
时间窗: 2 years
Fertilization rates will be retrospectively compared according to culture media in which sibling oocytes were incubated
次要结局
- Embryo Quality(2 years)