Significance of Immunohistochemical Expression of Wilms' Tumor 1 (WT1) in Colorectal Adenocarcinoma
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- detection of WT1 in colorectal adenocarcinoma
研究概览
简要总结
Colorectal carcinoma is a major bublic health problem. It is due to several genetic mutations. Wilms' tumor1 (WT1) gene is a tumor suppressor gene that has been mutated in several human tumors. Detection of WT1 in colorectal carcinoma may provide better future results.
详细描述
Colorectal adenocarcinoma represents one third of human caners worldwide. It results from cumulative genetic mutations. the incidence of colorectal carcinoma is rising, so, detecting new therapeutic modalities is important. Wilms' Tumor 1 (WT1) gene is a tumor suppressor gene. in certain tumors; WT1 acts as an oncogene, facilitating tumor growth and spread. WT1 is also involved in angiogenesis. we decided to detect expression of WT1 in 60 patients with colorectal adenocarcinoma and detect any association between WT1 expression and certain tumor oarameters as perineural invasion, lymphovascular invasion, lymph node metastasis.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •patients with colorectal adenocarcinoma.
排除标准
- •patients who received pre-operative neoadjuvant therapy.
研究组 & 干预措施
60 cases of colorectal carcinoma
60 formalin fixed, paraffin embedded tissue blocks belonged to patients with colorectal cancer. sections will be obtained and stained immunohistochemically with anti WT1 antibody.
干预措施: immunohistochemical staining with WT1 antibody (Biological)
结局指标
主要结局
detection of WT1 in colorectal adenocarcinoma
时间窗: 6 months
detection of WT1 in colorectal adenocarcinoma and correlate its expression in tumor cells with certain parameters as patients ages, sexes, tumor grade, lymph node metastasis, lymphovascular invasion and perineural invasion.
次要结局
未报告次要终点
研究者
Maisa Hashem Mohammed
Assistant Professor
Sohag University