Using GATA-3 and KRT 5/6 IHC markers for molecular phenotyping of bladder carcinoma: A Cross sectional study.
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 61
- 试验地点
- 1
- 主要终点
- To determine the immunohistochemical expression of GATA-3 and KRT 5/6 in all histologically confirmed cases of urinary bladder carcinoma and categorise them in basal and luminal phenotypes accordingly.
研究概览
简要总结
The study will be conducted in the Department of Pathology Rohilkhand Medical College and Hospital, Bareilly after obtaining approval from the Institutional Ethics Committee and will include all urinary bladder biopsy specimens received in the department in a one-year duration.
The received formalin-fixed biopsy specimens will be subjected to gross and microscopic examination as per standard protocol.
Haematoxylin and Eosin stains will be used to stain the previously cut paraffin-embedded tissues (4-5 microns).
Sections will be deparaffinized for immunohistochemistry, and Heat-Induced Epitome Retrieval (HIER) will be carried out using an EDTA buffer.
Tissue slices will be incubated with ready-to-use antibodies against IHC markers at a dilution of 1:100.
A 3,3’-diaminobenzene (DAB) detection kit, a hydrogen peroxide substrate, and a DAB chromogen will be utilized for the detection process. A DAB chromogen will be used to evaluate the markers’ antibody staining.
On IHC stained slides, observations will be taken and recorded.
In this study, the immunohistochemical markers GATA 3 and KRT 5/6 will be used.
IMMUNOHISTOCHEMICAL ANALYSIS: The prepared slides will be examined for positive or negative immunostaining using an optical microscope at x400 magnification.
STATISTICAL ANALYSIS: Data will be entered in SPSS (Statistical Package for social sciences); licensed version 23.0. Descriptive analysis will be done by calculating proportions, means and standard deviation. Appropriate statistical tests will be applied depending on the type and distribution of data. p <0.05 will be considered significant
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 0.00 Year(s) 至 90.00 Year(s)（—）
- 性别
- All
入选标准
- •All histopathologically confirmed cases of bladder carcinoma that will be received in our department.
排除标准
- •The biopsy reported as unsatisfactory and patients who are not willing to give consent.
- •Tissue is insufficient for reporting.
结局指标
主要结局
To determine the immunohistochemical expression of GATA-3 and KRT 5/6 in all histologically confirmed cases of urinary bladder carcinoma and categorise them in basal and luminal phenotypes accordingly.
时间窗: 7 days
次要结局
未报告次要终点
研究者
Mehnaz Munawar
Rohilkhand medical college and hospital