The Correlation of Sperm DNA Fragmentation With Blastocyst Grading and Its Implantation Potential
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 189
- 试验地点
- 1
- 主要终点
- Blastocyst morphological grading
研究概览
简要总结
Retrospective cohort study for 189 couples assigned to normal and abnormal Sperm DNA fragmentation (SDF) groups.To determine the effect of SDF on blastocyst's trophectoderm (TE), Inner cell mass (ICM) morphology grading, and implantation rate. Semen in both groups was processed by density gradient (DG).
详细描述
Purpose: to determine if sperm DNA fragmentation (SDF) has a correlation with Blastocyst's trophectoderm (TE) and Inner cell mass (ICM) grading, or with the implantation rate?
Method: Retrospective cohort study for 189 couples, assigned to normal SDF group (121 cases), and abnormal SDF group (68 cases). Semen in both groups was processed by double layer density gradient (DG).
Statistical analysis done using SPSS version 22.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- — 至 37 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •All cases in this study have to reach the blastocyst stage.
- •Female age ≤37 years old.
- •Male sperm count ≥ 5 million.
排除标准
- •Usage of sperm selection techniques.
- •Testicular aspiration or testicular biopsies.
- •Oocyte or sperm donation.
结局指标
主要结局
Blastocyst morphological grading
时间窗: 5-6 days after ICSI
Morphological grading of trophectoderm and Inner cell mass using Gardner's criteria 1999, from grade A to C ( A is the highest quality).
Implantation rate
时间窗: 6 weeks of pregnancy
Percentage of the implanted embryos number for each group / number of transferred embryos.
次要结局
- Blastulation rate(5-6 days of ICSI)
- Pregnancy rate(After 2 weeks of embryo transfer)
- Fertilization rate(16-19 hours of ICSI)