In Vitro Evaluation of Luteinized Granulosa Cells After Being Submitted to in Vitro Fertilization Protocols With Gonadotropin-Releasing Hormone (GnRH) Agonist and GnRH Antagonist
试验速览
- 阶段
- 不适用
- 入组人数
- 28
- 试验地点
- 2
- 主要终点
- Number of retrieved oocytes
研究概览
简要总结
This study is intended to evaluate ovarian cells after being submitted do different in vitro fertilization protocols. The investigators will analyze hormones secretion and intracellular mechanisms of these cells comparing the GnRH agonist protocol and the GnRH antagonist protocol.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 39 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Patient age limit: 39 years
- •Follicle Stimulating Hormone (FSH) < 10 micrometer (mU) / Liter
- •Antral follicular count > 12
- •Regular menses (25 to 35 days)
- •More than 3 oocytes after ovarian punction
- •Anti Mullerian between 1 e 3 ng/ml
排除标准
- •Patients submitted to intracytoplasmatic sperm injection (ICSI)
- •Ovarian mass or cyst
- •Clinical / endocrine pathologies
研究组 & 干预措施
GnRH Agonist
GnRH long agonist protocol of invitro fertilization
干预措施: randomization between the two GnRH analogues (agonist and antagonist) (Drug)
GnRH Antagonist
GnRH fixed antagonist protocol of in vitro fertilization
干预措施: randomization between the two GnRH analogues (agonist and antagonist) (Drug)
结局指标
主要结局
Number of retrieved oocytes
时间窗: After the ovarian punction
Immediately after the ovarian punction the number of retrieved oocytes will be evaluated
Evaluation of steroid secretion and enzyme expression in in vitro luteinized granulosa cells
时间窗: after 48h in media culture
evaluation of granulosa cells hormone secretion and enzyme expression in media culture 48 hours after ovarian punction
次要结局
- Subgroup analyses comparing FSH versus HMG protocols(after ovarian punction)
- number of embryos(3 to 5 days after ovarian punction)