Effect of Platelet Storage on Complement Activation
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 10
- 主要终点
- Platelets can be used after storage upto 5 days instead of using in the same day of donation without complement activation.
研究概览
简要总结
Platelet units are stored and complement factors are evaluated
详细描述
Ten units of platelets will be selected from the regular inventory of the Central Blood Transfusion Services/Assiut University Hospitals . All units of platelets will be collected, processed, and tested following the standard procedures.
Thesis units will be stored on a platelet rotator with the temperature controlled between 22 and 24c. Samples will be obtained using a sterile technique .
The investigators will take basal sample and samples on day 2,3,4 and 5. Complement components will be assayed : C4 (classical pathway), C3 (common pathway) and C5 (terminal pathway).
This evaluation will be done using ELISA technique
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Negative screening and confirmatory tests will be done for all platelet donors
排除标准
- •Use of aspirin or non steroidal anti-inflammatory drugs or medication affecting the platelets.
结局指标
主要结局
Platelets can be used after storage upto 5 days instead of using in the same day of donation without complement activation.
时间窗: 5 days
次要结局
未报告次要终点
研究者
Shimaa Abdeleem
Doctor
Assiut University