The Effect of Modulating Hepatic Glycogen Content on Hepatic Fat Oxidation
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 11
- 试验地点
- 2
- 主要终点
- Hepatic fat oxidation
研究概览
简要总结
Excessive fat in the liver is associated with impairments in metabolic health. Low levels of DNL and high levels of hepatic fat oxidation are considered to be protective.
A decrease in glycogen stores has been causally linked to improved whole body fat oxidation. Also on an organ level, it is suggested that hepatic fat oxidation is stimulated by low hepatic glycogen stores. Next to hepatic fat oxidation, DNL may be influenced by hepatic glycogen stores. Some studies have shown that prolongation of fasting time lowers hepatic glycogen content. It is therefore hypothesized that prolonging fasting time will lower glycogen content and thereby increases fat oxidation and decreases DNL in the liver. To this end, hepatic fat oxidation (plasma marker beta-hydroxybutyrate), de novo lipogenesis, hepatic glycogen content and intrahepatic fat content, will be measured upon a short overnight fast and an extended overnight fast in 13 overweight/obese subjects with hepatic steatosis.
详细描述
Rationale:
Excessive fat in the liver is associated with impairments in metabolic health. Hepatic lipid storage can originate from several metabolic pathways, including de novo lipogenesis (DNL). On the other hand, there are several metabolic pathways that can decrease hepatic fat storage, such as hepatic fat oxidation. Therefore, low levels of DNL and high levels of hepatic fat oxidation are considered to be protective.
A decrease in glycogen stores has been causally linked to improved whole body fat oxidation. Also on an organ level, it is suggested that hepatic fat oxidation is stimulated by low hepatic glycogen stores. Furthermore, whole body glycogen levels were also linked to DNL and in situations with increased whole body glycogen levels in animal models rates of DNL were also high. Therefore, next to hepatic fat oxidation, DNL may be influenced by hepatic glycogen stores.
Some studies have shown that prolongation of fasting time lowers hepatic glycogen content. It is therefore hypothesized that prolonging fasting time will lower glycogen content and thereby increases fat oxidation and decreases DNL in the liver.
Objective:
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 45 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Signed informed consent
- •Caucasian (people will be excluded when having a ≥50% racial African/Asian background)
- •Male or postmenopausal female
- •Aged 45-75 years at start of the study
- •Body mass index (BMI) 27 - 38 kg/m2
- •Stable dietary habits (no weight loss or gain >3kg in the past 3 months)
- •Sedentary lifestyle (not more than 2 hours of sports per week)
- •Liver fat ≥5% as determined by 1H-MRS
排除标准
- •Type 2 diabetes
- •Active diseases (cardiovascular, diabetes, liver, kidney, cancer or other)
- •Contra-indication for MRI (which can be found in appendix II)
- •Alcohol consumption of >2 servings per day
- •Regular smoking (>5 cigarettes per day)
- •No use of medication interfering with investigated study parameters (as determined by responsible physician)
结局指标
主要结局
Hepatic fat oxidation
时间窗: 5 hours
measured as plasma BHB levels
次要结局
- De novo lipogenesis (DNL)(20 hours)