Deciphering the Effect of Moderate Wine Consumption on Healthy Aging Through Postprandial Extracellular Vesicles
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 8
- 试验地点
- 1
- 主要终点
- Concentration of circulating extracellular vesicles
研究概览
简要总结
This study aims to investigate how moderate wine consumption influences circulating extracellular vesicles (EVs) in healthy adults. EVs are small particles released by cells that carry proteins, lipids, and genetic material, and play important roles in communication between cells. Participants will consume a single serving of red or white wine, and blood samples will be collected before and after consumption to study changes in the composition and function of EVs. The study will also assess how these EVs affect vascular, immune, and brain-related cells. The results are expected to improve our understanding of how moderate wine intake contributes to cardiovascular and brain health.
详细描述
Dietary interventions have been consistently proposed as a part of a comprehensive strategy to lower the incidence and severity of atherosclerosis and coronary vascular disease. According to the Dietary Guidelines for Americans 2015-2020, moderate red wine consumption-defined as up to two units of alcohol per day for men and up to one unit of alcohol per day for women-significantly reduces the risk of cardiovascular disease (CVD). Wine is a complex matrix consisting primarily of water (86%) and ethyl alcohol (12%), as well as other different molecules, such as polyphenols, organic acids, tannins, compound minerals, vitamins and biologically active compounds. While most of the nutritional studies have focused on elucidating the mechanisms by which biologically active compounds directly affect cardiovascular-related events, little or nothing is known about the regulatory effect of wine consumption on extracellular vesicles (EVs). EVs are small phospholipid particles that convey molecular bioactive cargoes and play essential roles in intercellular communication and, hence, a multifaceted role in health and disease. Recent advances in research on EVs have significantly enhanced their potential as therapeutic agents for neurological diseases. Owing to their therapeutic properties and ability to cross the blood-brain barrier (BBB), extracellular vesicles are recognized as promising drug delivery vehicles (e.g. polyphenols) for various neurological conditions. For the first time, the purpose of this project is to establish whether the moderate wine consumption may alter the structure, cargo, and functionality of postprandial EVs. In the precision nutrition era, we expect to offer a new insight on EVs and their relationship with wine through the following objectives: 1) To map changes in the lipidome, proteome, polyphenolic cargo, and functional properties of circulating EVs in healthy subjects both at fasting and at postprandial state upon a challenge of a one unit of red or white wine; 2) To analyse the effect of postprandial EVs on atherosclerotic-related events in endothelial and immune cells. 3) To analyse the effect of postprandial EVs on healthy ageing in blood-brain barrier and microglial cells. Collectively, this project will provide fundamental insight into EV biology and remarks the clinical and functional relevance and consequences of moderate wine consumption in health and disease.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 35 Years 至 36 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Healthy adult men and women aged 35 to 65 years.
- •Body Mass Index (BMI) between 18.5 and 29.9 kg/m².
- •Non-smokers or ex-smokers for at least 12 months.
- •Moderate alcohol consumers, defined as ≤2 units/day for men and ≤1 unit/day for women.
- •Normal fasting glucose and lipid profile at screening.
- •Willing and able to refrain from alcohol, polyphenol-rich foods, and intense exercise for 48 hours before each study visit.
- •Able to understand the study procedures and provide written informed consent.
排除标准
- •History or clinical evidence of cardiovascular, hepatic, renal, thyroid, gastrointestinal, or metabolic diseases (including diabetes, dyslipidemia, or hypertension).
- •Use of medications or supplements known to affect glucose, lipid, or inflammatory metabolism (e.g., statins, corticosteroids, anti-inflammatory drugs).
- •Pregnancy or breastfeeding.
- •Recent blood donation (within the last 3 months) or planned blood donation during the study period.
- •Major weight change (>5% of body weight) within the last 3 months.
- •Participation in another clinical or biomedical study within the previous 3 months.
- •Known allergy or intolerance to wine, alcohol, or its components (e.g., sulfites).
- •History of alcohol abuse or inability to abstain from alcohol outside the study context.
- •Reluctance to receive information about incidental health findings arising from the study.
- •Any condition judged by the investigators to limit compliance or increase study risk (e.g., psychiatric disorders, inability to adhere to fasting requirements).
研究组 & 干预措施
Red Wine
Participants will consume red wine (Cabernet Sauvignon) at a dose of 4 mL per kg body weight within 15 minutes. Blood samples will be collected at fasting (0 h), postprandial peak (1-2 h), and post-peak (6 h).
干预措施: Red Wine (Dietary Supplement)
White Wine
Participants will consume white wine (Chardonnay) at a dose of 4 mL per kg body weight within 15 minutes. Blood samples will be collected at fasting (0 h), postprandial peak (1-2 h), and post-peak (6 h).
干预措施: White Wine (Dietary Supplement)
Water (Control)
Participants will consume water (4 mL per kg body weight) within 15 minutes, serving as the control condition. Blood samples will be collected at fasting (0 h), postprandial peak (1-2 h), and post-peak (6 h).
干预措施: Water (Dietary Supplement)
结局指标
主要结局
Concentration of circulating extracellular vesicles
时间窗: Baseline (0 hours), postprandial peak (2 hours after beverage intake), and post-peak (6 hours after beverage intake).
Concentration of plasma-derived extracellular vesicles measured after acute intake of red wine, white wine, or water. Extracellular vesicles will be isolated by immunoaffinity methods and quantified using nanoparticle-based analysis. Results will be expressed as particles per milliliter (particles/mL).
Mean size of circulating extracellular vesicles
时间窗: Baseline (0 hours), postprandial peak (2 hours after beverage intake), and post-peak (6 hours after beverage intake).
Mean diameter of plasma-derived extracellular vesicles measured after acute intake of red wine, white wine, or water. Extracellular vesicle size will be assessed using dynamic light scattering. Results will be expressed in nanometers (nm).
次要结局
- Postprandial changes in extracellular vesicle proteome(Baseline (0 hours), postprandial peak (2 hours after beverage intake), and post-peak (6 hours after beverage intake).)
- Postprandial changes in extracellular vesicle lipidome(Baseline (0 hours), postprandial peak (2 hours after beverage intake), and post-peak (6 hours after beverage intake).)
- Change from baseline in plasma glucose concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma insulin concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma triglyceride concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma total cholesterol concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma HDL cholesterol concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma LDL cholesterol concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma interleukin-6 (IL-6) concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma tumor necrosis factor alpha (TNF-α) concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma interleukin-1 beta (IL-1β) concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Change from baseline in plasma monocyte chemoattractant protein-1 (MCP-1/CCL2) concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake)
- Transendothelial electrical resistance (TEER) in an in vitro human blood-brain barrier model after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; TEER assessed within 72 hours after EV isolation)
- Claudin-5 (CLDN5) protein expression in an in vitro human blood-brain barrier model after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; protein expression assessed within 72 hours after EV isolation)
- Occludin (OCLN) protein expression in an in vitro human blood-brain barrier model after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; protein expression assessed within 72 hours after EV isolation)
- Interleukin-6 (IL-6) concentration in microglial culture supernatant after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; cytokines assessed within 72 hours after EV isolation)
- Tumor necrosis factor alpha (TNF-α) concentration in microglial culture supernatant after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; cytokines assessed within 72 hours after EV isolation)
- IL1B mRNA expression in microglia after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; gene expression assessed within 72 hours after EV isolation)
- Endothelial VCAM-1 protein expression after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; assays conducted within 48 hours after EV isolation)
- Polyphenolic content of extracellular vesicles after wine intake(Baseline (0 hours), postprandial peak (2 hours after beverage intake), and post-peak (6 hours after beverage intake).)
- Spearman correlation between EV cargo score and change from baseline in plasma glucose concentration(Baseline (0 hours), 2 hours, and 6 hours after beverage intake (plasma glucose); EVs isolated at baseline (0 hours) and 2 hours after beverage intake)
- Endothelial ICAM-1 protein expression after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; assays conducted within 48 hours after EV isolation)
- Nitric oxide (NO) production in endothelial cell culture supernatant after exposure to postprandial extracellular vesicles(EVs isolated at baseline (0 hours) and 2 hours after beverage intake; assays conducted within 48 hours after EV isolation)
研究者
Sergio Montserrat de la Paz
Full Professor
University of Seville