The Function of Biphasic Sleep in Infants

Four days prior to the first session, an experimenter will meet the caregiver and infant (at the lab or at their home) to review procedures and complete the informed consent process. The infant will be fitted with the actigraph watch on the ankle. The caregiver will be given instructions on use of the actigraph watch and be left with questionnaires to complete (e.g., sleep diary, behavioral questionnaires). Four days later, the caregiver and infant will come to the sleep lab for their initial session. In wave 1 (9 months), caregiver-infant dyads will be scheduled to arrive ~1 hr prior to the infant's typical morning nap time. In later waves, if the infant is reported to have eliminated their morning nap, they will be scheduled to arrive ~1 hr prior to when the nap typically occurred before they transitioned out of the morning nap. Infants will be semi-randomly assigned to either the nap or wake condition. Following acclimation to the lab, the infant will be fitted with the polysomnography (electrode) cap. The infant will then complete the encoding and immediate recall phases of the deferred imitation (memory) task. The infant will then be either wake or nap promoted or allowed to follow their normal morning routine. Approximately 90 mins later (following nap waking and time to recover from sleep inertia), the deferred imitation task (recall) will take place.

The caregiver and infant will then have free time (lunch, playtime), with instructions to return to the lab (if they choose to leave; they are also offered access to the lab kitchen and lounge area) ~40 mins before the infant's typical afternoon nap. They will also be instructed to: (1) avoid letting the infant nap and report any sleep that occurred (this will also be monitored with actigraphy); (2) not partake in any unusually stimulating activities; and (3) to follow a routine feeding schedule during this time. When the dyad returns to the lab, the infant will again be equipped with the polysomnography cap and complete the encoding and immediate recall phases with a new set of items. The infant will then either be wake or nap promoted or allowed to follow their normal afternoon nap routine. Approximately 90 mins later (following nap and time to recover from sleep inertia), the delayed recall phase will take place. The infant and caregiver will return to the lab one week later to complete the other condition. The procedures will be repeated at 12 (>12 months and <13 months) and 15 months (>15 months and <16 months).