Effects of Acute Exercise on (Myokine) Gene Expression in Human Skeletal Muscle
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 12
- 试验地点
- 2
- 主要终点
- Gene expression changes in skeletal muscle from baseline to after exercise
研究概览
简要总结
Rationale: Proteins released from muscle during and shortly after exercise, often referred to as myokines, may be central to our understanding of the cross-talk during and after exercise between skeletal muscles and other organs, in particular the liver. So far only a few myokines are identified (e.g. IL-6, IL-8, IL-15, TNF-alpha). Taking into account the role of these several known myokines in developing insulin resistance, revealing new putative myokines might provide valuable information and a direction for future research on the pathogenesis and treatment of type 2 diabetes mellitus.
Objective: The objective of the present study is to identify novel myokines, expression of which is altered in skeletal muscle after a single bout of exercise.
Study design: experimental study. Study population: Ten healthy, male subjects between 40 and 60 years of age and BMI < 30 kg/m2, will participate in this study.
Intervention: A single exercise bout that consists of one hour one-legged cycling on a adapted recumbent cycle ergometer at a submaximal rate. The non-exercising leg will serve as control for the exercising leg.
Main study outcomes: Main study outcomes include upregulation of genes in skeletal muscle after exercise (with a focus on genes encoding myokines) and changes of blood plasma levels of selected proteins after exercise.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 40 Years 至 60 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Age 40-60 years
- •Male gender
- •BMI < 30 kg/m2
排除标准
- •Exercising regularly (> 2 times a week, > 3 hour in total per week)
- •Unstable body weight (weight gain or loss > 3 kg in the past three months)
- •Following, or have recently followed a (weight-loss) diet
- •Donated or intended to donate blood 2 months before until two months after the study
- •Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease)
- •Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg
- •Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate).
- •Use of antithrombotic therapy (marcoumar, sintromitis).
- •Diagnosed diabetes mellitus type 1 or
- •Drugs or alcohol abuse ( > 3 glasses of alcoholic beverages a day).
- •(Chronic) injuries of the locomotor system that can interfere with the intervention
- •Participated in another study within the last six months
结局指标
主要结局
Gene expression changes in skeletal muscle from baseline to after exercise
时间窗: T = 0 and T =1
Gene expression is measured using whole genome Affymatrix microarrays Muscle tissue will be collected at time points T = 0 (before exercise) and T = 1 (directly after exercise). Special focus is on the changes in genes coding for myokines. Up to 5 genes will be selected for follow-up analysis.
Changes in plasma levels of selected proteins
时间窗: T = 0, T =1 and T = 3
Selected proteins will be analyzed by ELISA assays or western blot analysis, depending on ELISA availability at T = 0, T = 1 and T = 3 (2 hours post-exercise). The selection of the proteins is based on significance, the robustness of induction (\>80% of subjects showing induction) and the magnitude of the induction ( mean fold change \> 2)
次要结局
- PBMC gene expression changes before and after exercise(T = 0, T = 1 and T =3)
- Routine plasma level changes before and after exercise(T = 0, 1 and 3)
- Heart rate changes, baseline compared with exercise(During the intervention)