Effects of a 4 Week Cold Tub vs Cold Shower Immersion Intervention on Immune Function, Sleep Quality, Mental Health and Well-being, Gut Health, and Muscular Strength in Healthy, Physically Active Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 37
- 试验地点
- 1
- 主要终点
- C-Reactive Protein
研究概览
简要总结
The purpose of this clinical trial is to explore the effects of a 4 week cold-water immersion (CWI) intervention on measures of immune function, sleep quality, mental health and well-being, and muscular strength. The main questions it aims to answer are:
- Does a 4 week CWI intervention improve measures of immune function, sleep quality, mental health and well-being, and muscular strength?
- Are there any differences in these measures when comparing cold tubs versus cold showers?
Researchers will compare chronic use of cold tubs to cold showers to see if cold water immersion may improve overall immune health and well-being. Participants will:
- Undergo 4 weeks of Cold Tub or Cold Shower interventions at a frequency of 3 times a week for 4 minutes, if placed in the CWI intervention groups
- Undergo testing measures at the two pre- and post- intervention time points, if placed in the healthy control group or the CWI intervention groups
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Males and Females
- •Aged 18-45
排除标准
- •Currently practices CWI for [>3 times in the last month]
- •Pregnant or planning to become pregnant
- •Chronic use of anti-inflammatory medication
- •Prior history of chronic disease conditions:
- •Cardiovascular Disease
- •Raynaud's Disease
- •Severe and untreated anxiety or depression
研究组 & 干预措施
Cold Tub Immersion Group (CI)
Participants will utilize the research cold tub (10 ºC, 4-minutes, 3 times a week), following a standardized protocol (see intervention description).
干预措施: Cold Water Immersion (CWI) - Cold Tub Immersion (CI) (Other)
Cold Shower Group (CS)
Participants will utilize the research shower area (10 ºC, 4-minutes, 3 times a week), following a standardized protocol (see intervention description).
干预措施: Cold Water Immersion (CWI) - Cold Shower (CS) (Other)
Control Group (CO)
Participants will not undergo any cold tub or cold shower intervention.
干预措施: Control Group (CO) (Other)
结局指标
主要结局
C-Reactive Protein
时间窗: Visit 1A (Week 1) and Visit 2A (Week 4): 15 minutes for blood draw. The assay itself takes 3 hours.
Approximately 60mL of whole blood will be collected into serum separator tubes (Beckton Dickinson, East Rutherford, NJ, USA), allowed to clot for 30-min at room temperature, then centrifuged at 2000 RPM for 15 min. The serum will be pipetted into 1.5 mL microcentrifuge tubes (Eppendorf AG, Hamburg, Germany) and immediately stored in a -80 °C freezer. Serum concentrations of CRP will be determined with a commercially available enzyme-linked immunosorbent assay (ALPCO Diagnostics, Salem, NH, USA). Microplates will be read with an ELx800 BioTek microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) at the recommended wavelength of 450 nanometers.
Interleukin-6 (IL-6)
时间窗: Visit 1A (Week 1) and Visit 2A (Week 4): 15 minutes for blood draw. The assay itself takes 3 hours.
Approximately 60mL of whole blood will be collected into serum separator tubes (Beckton Dickinson, East Rutherford, NJ, USA), allowed to clot for 30-min at room temperature, then centrifuged at 2000 RPM for 15 min. The serum will be pipetted into 1.5 mL microcentrifuge tubes (Eppendorf AG, Hamburg, Germany) and immediately stored in a -80 °C freezer. Serum concentrations of IL-6 will be determined with a commercially available enzyme-linked immunosorbent assay (Thermo Fisher Scientific, Waltham, MA, USA). Microplates will be read with an ELx800 BioTek microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) at the recommended wavelength of 450 nanometers.
次要结局
- Pittsburgh Sleep Quality Index (PSQI)(Visit 1A (Week 1) and 2A (Week 4): 10 minutes each)
- Depression Anxiety and Stress Scales (DASS-21)(Visit 1A (Week 1) and 2A (Week 4): 10 minutes each)
- Handgrip Dynamometry(Visit 1B (Week 1) and Visit 2B (Week 4): 10 minutes each)
研究者
Laura Stewart
Professor
University of Northern Colorado